Detailed information of TA system TA236089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 299557..300275
• Replicon: plasmid pRSpK102a
• Accession: NZ_CP092435
• Organism: Rhizobium sp. K102

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: MHI61_RS05340
• Protein ID: WP_097606618.1
• Coordinates: 299892..300275 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: MHI61_RS05335
• Protein ID: WP_097606619.1
• Coordinates: 299557..299895 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHI61_RS05310 (MHI61_05315)	295050..295754	+	705	WP_239789501.1	ABC transporter permease subunit	-
MHI61_RS05315 (MHI61_05320)	295780..296556	+	777	WP_040111353.1	ATP-binding cassette domain-containing protein	-
MHI61_RS05320 (MHI61_05325)	296553..298043	+	1491	WP_239789503.1	histidine ammonia-lyase	-
MHI61_RS05325 (MHI61_05330)	298052..298597	-	546	WP_239789505.1	hypothetical protein	-
MHI61_RS05330 (MHI61_05335)	298757..299494	+	738	WP_097606620.1	phosphatase PAP2 family protein	-
MHI61_RS05335 (MHI61_05340)	299557..299895	-	339	WP_097606619.1	helix-turn-helix transcriptional regulator	Antitoxin
MHI61_RS05340 (MHI61_05345)	299892..300275	-	384	WP_097606618.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MHI61_RS05345 (MHI61_05350)	300441..303002	-	2562	WP_239789506.1	FAD-dependent oxidoreductase	-
MHI61_RS32600	303111..303350	-	240	WP_275593068.1	hypothetical protein	-
MHI61_RS05350 (MHI61_05355)	303263..303502	-	240	WP_009987796.1	hypothetical protein	-
MHI61_RS05355 (MHI61_05360)	303514..303774	-	261	WP_010032482.1	hypothetical protein	-
MHI61_RS05360 (MHI61_05365)	303775..304092	-	318	WP_239789507.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..445874	445874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-90)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14716.84 Da
• Isoelectric Point: 9.8531

>T236089 WP_097606618.1 NZ_CP092435:c300275-299892 [Rhizobium sp. K102]
MENLPRPTRPLLWIASSRKDYAEFPPKVQEAFGFELFLAQTGQHPPSAKMLKGLGNGIVELVEDHDSDTYRAVYTVRFET
AVYVLHCFKKKSKRGIKTPQSDIELIKHRFRDAEAHYAAKQNEERKR

Nucleotide

• Length: 384 bp

>T236089 NZ_LR135297:494393-494734 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12471.27 Da
• Isoelectric Point: 7.5738

>AT236089 WP_097606619.1 NZ_CP092435:c299895-299557 [Rhizobium sp. K102]
MKTEHVEVHAGHPNIFAELGLPDAENHFLKAQIVAALHRLVNERKLNQAKAGTIMGITQPEVSRLFRGHFREYSVERLMS
FLTAFNQDVEIVVRPHAKAGEAGNITFNAVTA

Nucleotide

• Length: 339 bp

>AT236089 NZ_LR135297:494163-494393 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG