Detailed information of TA system TA236077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 65362..65906
• Replicon: plasmid unnamed1
• Accession: NZ_CP092424
• Organism: Mycobacterium lentiflavum strain ATCC 51985

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MJO58_RS27770
• Protein ID: WP_084954129.1
• Coordinates: 65362..65640 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0E4H3K5
• Locus tag: MJO58_RS27775
• Protein ID: WP_061559515.1
• Coordinates: 65637..65906 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJO58_RS27750 (MJO58_27575)	60678..61187	-	510	Protein_54	sulfite exporter TauE/SafE family protein	-
MJO58_RS27755 (MJO58_27580)	61715..62395	+	681	WP_061559513.1	response regulator transcription factor	-
MJO58_RS27760 (MJO58_27585)	63238..64658	-	1421	Protein_56	IS3 family transposase	-
MJO58_RS27765 (MJO58_27590)	64987..65148	+	162	WP_239723505.1	hypothetical protein	-
MJO58_RS27770 (MJO58_27595)	65362..65640	-	279	WP_084954129.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJO58_RS27775 (MJO58_27600)	65637..65906	-	270	WP_061559515.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MJO58_RS27780 (MJO58_27605)	66685..67140	+	456	WP_061559516.1	hypothetical protein	-
MJO58_RS27785 (MJO58_27610)	67232..67801	-	570	WP_061559517.1	hypothetical protein	-
MJO58_RS27790 (MJO58_27615)	67798..68319	-	522	WP_239723506.1	helix-turn-helix transcriptional regulator	-
MJO58_RS27795 (MJO58_27620)	68673..68777	+	105	WP_082811763.1	DUF1330 domain-containing protein	-
MJO58_RS27800 (MJO58_27625)	68840..69028	-	189	Protein_64	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..224738	224738
	-	flank	IS/Tn	-	-	63238..64260	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-90)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10587.13 Da
• Isoelectric Point: 10.5216

>T236077 WP_084954129.1 NZ_CP092424:c65640-65362 [Mycobacterium lentiflavum]
VTENDDAWSVQVSPAAARALERLPHKVAAAIAEFITVALPADPLRMSKPLRFQFEGWRVARRGDYRVTFRILSDDNVLYV
GRIEHRAHVYRR

Nucleotide

• Length: 279 bp

>T236077 NZ_LR135279:c218535-218263 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9530.58 Da
• Isoelectric Point: 4.4262

>AT236077 WP_061559515.1 NZ_CP092424:c65906-65637 [Mycobacterium lentiflavum]
MKQVPLSEAKDKLSALVDEADTTHEIIQITRHGRVAAVIMSADDLDSLNETLHALGTPGLADELAQADADYAAGNTVSGN
DIRRRYGLQ

Nucleotide

• Length: 270 bp

>AT236077 NZ_LR135279:c218798-218535 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E4H3K5