Detailed information of TA system TA236074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3590022..3591058
• Replicon: chromosome
• Accession: NZ_CP092419
• Organism: Leptolyngbya boryana IU 594

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MCP04_RS17235
• Protein ID: WP_017288215.1
• Coordinates: 3590022..3590588 (-)
• Length: 189 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A1Z4JIQ6
• Locus tag: MCP04_RS17240
• Protein ID: WP_017288216.1
• Coordinates: 3590594..3591058 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCP04_RS17210 (MCP04_17210)	3585043..3585372	+	330	WP_017288210.1	hypothetical protein	-
MCP04_RS17215 (MCP04_17215)	3586776..3587873	-	1098	WP_017288211.1	PAS domain-containing protein	-
MCP04_RS17220 (MCP04_17220)	3588367..3588717	-	351	WP_017288212.1	hypothetical protein	-
MCP04_RS17225 (MCP04_17225)	3588755..3588964	+	210	WP_144056198.1	hypothetical protein	-
MCP04_RS17230 (MCP04_17230)	3589017..3589649	-	633	WP_017288213.1	hypothetical protein	-
MCP04_RS17235 (MCP04_17235)	3590022..3590588	-	567	WP_017288215.1	PIN domain-containing protein	Toxin
MCP04_RS17240 (MCP04_17240)	3590594..3591058	-	465	WP_017288216.1	helix-turn-helix domain-containing protein	Antitoxin
MCP04_RS17245 (MCP04_17245)	3591152..3591307	-	156	WP_154660829.1	hypothetical protein	-
MCP04_RS17250 (MCP04_17250)	3591442..3591645	-	204	WP_081614741.1	hypothetical protein	-
MCP04_RS17260 (MCP04_17260)	3592122..3594830	-	2709	WP_017288219.1	type I DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• SinI-like, DNA-binding domain (83-128)

Sequences

Toxin

Protein

• Length: 189 a.a.
• Molecular weight: 21641.89 Da
• Isoelectric Point: 5.6857

>T236074 WP_017288215.1 NZ_CP092419:c3590588-3590022 [Leptolyngbya boryana IU 594]
MSNFTAVYDACVLYPAPLRDFLMELALTDLFRARWSESIHQEWIRNVLKSRPDLSAEQLNKTKELMNSHVRDSVVTNYET
LIPAIELPDPDDRHVLAAAIRCNASVIVTFNLKDFPADTLKLYDIDAQHPDEFILYLLDLKPLAVCTAAERQRQRLKKPP
KDPEEYLGTLLRQGLPQTVSRLRESCYL

Nucleotide

• Length: 567 bp

>T236074 NZ_LR135261:c48475-48125 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17200.86 Da
• Isoelectric Point: 6.2467

>AT236074 WP_017288216.1 NZ_CP092419:c3591058-3590594 [Leptolyngbya boryana IU 594]
MTITLQKSSPPTLEDIRLAKESSSRLARSIPSHESTSKISVIQGEATGETIVLPTAALQMLVEILAQMAQGNIVTLFPIH
AELTTQEAADLLKVSRPYLVKLLEDKALPFRKVGKHRRILFQDLMEYKNREDSLRSNALDELVAQAQELNMGYE

Nucleotide

• Length: 465 bp

>AT236074 NZ_LR135261:c48732-48469 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z4JIQ6