Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 2063931..2064493 Replicon chromosome
Accession NZ_CP092419
Organism Leptolyngbya boryana IU 594

Toxin (Protein)


Gene name vapC Uniprot ID A0A1Z4JEA4
Locus tag MCP04_RS09490 Protein ID WP_017286620.1
Coordinates 2064152..2064493 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1Z4JEC0
Locus tag MCP04_RS09485 Protein ID WP_017286619.1
Coordinates 2063931..2064155 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCP04_RS09465 (MCP04_09465) 2060025..2060189 - 165 WP_017286615.1 hypothetical protein -
MCP04_RS09470 (MCP04_09470) 2060595..2061284 - 690 WP_017286616.1 glycoside hydrolase family protein -
MCP04_RS09475 (MCP04_09475) 2061350..2062984 - 1635 WP_017286617.1 ABC transporter ATP-binding protein -
MCP04_RS09480 (MCP04_09480) 2063034..2063720 - 687 WP_017286618.1 SEC59/DGK1/VTE5 family protein -
MCP04_RS09485 (MCP04_09485) 2063931..2064155 + 225 WP_017286619.1 DUF433 domain-containing protein Antitoxin
MCP04_RS09490 (MCP04_09490) 2064152..2064493 + 342 WP_017286620.1 DUF5615 family PIN-like protein Toxin
MCP04_RS09495 (MCP04_09495) 2064538..2065359 - 822 WP_017286621.1 slipin family protein -
MCP04_RS09500 (MCP04_09500) 2065545..2066290 - 746 Protein_1880 IS1 family transposase -
MCP04_RS09505 (MCP04_09505) 2066343..2067227 - 885 WP_239741293.1 branched-chain amino acid ABC transporter permease -
MCP04_RS09510 (MCP04_09510) 2067224..2068174 - 951 WP_017286623.1 branched-chain amino acid ABC transporter permease -
MCP04_RS09515 (MCP04_09515) 2068216..2069400 - 1185 WP_017286624.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(4-79)

Antitoxin

Protein of unknown function DUF433

(8-63)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12378.18 Da        Isoelectric Point: 4.1314

>T236072 WP_017286620.1 NZ_CP092419:2064152-2064493 [Leptolyngbya boryana IU 594]
VTTIWIDAHLSPVIATWITGRFGITAIALRDIGLRDAEDPEIFEAAKAQGVIFMTKDSDFVDLVERLEAPPQIIWLTCGN
TSNARLQEILSSTLPDALELLKSGEKLVEISGE

Download         Length: 342 bp

>T236072 NZ_LR135259:96197-96538 [Enterococcus faecium]
ATGACTGAAGAAAAAAATTACATTCCTAAAAAGGGAGATATTGTTTGGATTGATTTTGATCCTTCTGCAGGAAAAGAGAT
TCAAAAGCGGCGTCCTGGATTAGTTGTTTCTAGATACGAGTTTAATCGCAAAACAATGTTTGCAGTTATTTGTCCGATTA
CCTCTACTATAAGAAATTTTCCGACACGCTATTCATTACCTACCGAATTAGACACTACTGGACAAATACTTATTTCTCAA
TTGAAGTCGCTCGATTTCAATGAGAGAAAACTTAAAAAAATTGAATCCTTACCTTTACAAGATATGGCGAAAATCGATCA
AATGATTGAATATATATTTTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8186.57 Da        Isoelectric Point: 7.8330

>AT236072 WP_017286619.1 NZ_CP092419:2063931-2064155 [Leptolyngbya boryana IU 594]
MSDLLKRITVNPKQCGGRPCIRGMRIRVSDVLDLFAAGLSAEQILEELPDLERDDLRAALAYASRKVNHPVLVA

Download         Length: 225 bp

>AT236072 NZ_LR135259:95967-96197 [Enterococcus faecium]
ATGCTTATTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGATTACTTTGCCTTCTGATAACGGAAAGAAGCCTTCGGA
AAATAAAGAATATATAGTTATCTATTCTGATGATGGAACAATTACTTTGGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAAGCGGAGTATTACGAAAAAGATGAATGGGAAGATTTGTCTCCTGAAGGAAGAGAGATTTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z4JEA4

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z4JEC0

References