236070

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 1311910..1312487 Replicon chromosome
Accession NZ_CP092419
Organism Leptolyngbya boryana IU 594

Toxin (Protein)


Gene name mazE Uniprot ID A0A1Z4JC99
Locus tag MCP04_RS06075 Protein ID WP_017285931.1
Coordinates 1311910..1312236 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A1Z4JCF0
Locus tag MCP04_RS06080 Protein ID WP_017285932.1
Coordinates 1312224..1312487 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCP04_RS06040 (MCP04_06040) 1307094..1307468 - 375 WP_017285924.1 hypothetical protein -
MCP04_RS06045 (MCP04_06045) 1307557..1307826 - 270 WP_017285925.1 hypothetical protein -
MCP04_RS06050 (MCP04_06050) 1307886..1308224 - 339 WP_017285926.1 hypothetical protein -
MCP04_RS06055 (MCP04_06055) 1308627..1309904 + 1278 WP_017285927.1 adenosylhomocysteinase -
MCP04_RS06060 (MCP04_06060) 1309954..1310298 - 345 WP_017285928.1 DUF86 domain-containing protein -
MCP04_RS06065 (MCP04_06065) 1310288..1310623 - 336 WP_017285929.1 nucleotidyltransferase family protein -
MCP04_RS06070 (MCP04_06070) 1310721..1311725 + 1005 WP_017285930.1 GUN4 domain-containing protein -
MCP04_RS06075 (MCP04_06075) 1311910..1312236 - 327 WP_017285931.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MCP04_RS06080 (MCP04_06080) 1312224..1312487 - 264 WP_017285932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MCP04_RS06085 (MCP04_06085) 1312612..1313412 + 801 WP_017285933.1 mechanosensitive ion channel family protein -
MCP04_RS06090 (MCP04_06090) 1313764..1314609 + 846 WP_017285934.1 3-deoxy-7-phosphoheptulonate synthase -
MCP04_RS06095 (MCP04_06095) 1315066..1316808 - 1743 WP_017285935.1 NFACT RNA binding domain-containing protein -
MCP04_RS06100 (MCP04_06100) 1316869..1317114 + 246 WP_017285936.1 DUF370 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-101)

Antitoxin

SpoVT-AbrB domain

(12-49)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12201.35 Da        Isoelectric Point: 8.5508

>T236070 WP_017285931.1 NZ_CP092419:c1312236-1311910 [Leptolyngbya boryana IU 594]
MAMVVRRFDVFLVQLDPTVGSEIQKTRPCIIVSPDEMNRLLKTVLIAPLTSVRRNFPFRVDCQFQGKAGQIVLDQIRAVD
KTRLVQFLGQIGEEQRALLDTIADLFAA

Download         Length: 327 bp

>T236070 NZ_LR135258:191116-191382 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9789.91 Da        Isoelectric Point: 4.2815

>AT236070 WP_017285932.1 NZ_CP092419:c1312487-1312224 [Leptolyngbya boryana IU 594]
MSGTIRTRLVKFGNSQGIRIPKLLLEQSGIQSEVEIEVEGNRLIIRPVSHPRAGWEAAIDAEIAKSGDEGLLDDETSTQW
DETEWQW

Download         Length: 264 bp

>AT236070 NZ_LR135258:190853-191116 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z4JC99

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z4JCF0

References