Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1233413..1234044 Replicon chromosome
Accession NC_012207
Organism Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag JTY_RS05880 Protein ID WP_003405820.1
Coordinates 1233413..1233724 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag JTY_RS05885 Protein ID WP_003405836.1
Coordinates 1233724..1234044 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTY_RS05860 1228895..1230319 - 1425 WP_003405805.1 class II fumarate hydratase -
JTY_RS05865 1230350..1231438 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
JTY_RS05870 1231494..1232138 + 645 WP_174813088.1 DUF4245 domain-containing protein -
JTY_RS05875 1232145..1233301 - 1157 Protein_1140 AI-2E family transporter -
JTY_RS05880 1233413..1233724 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
JTY_RS05885 1233724..1234044 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
JTY_RS20895 1234054..1235579 + 1526 Protein_1143 carboxylesterase/lipase family protein -
JTY_RS05900 1235597..1236709 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
JTY_RS05905 1236719..1236976 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
JTY_RS05910 1236966..1238213 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
JTY_RS05915 1238210..1238848 - 639 WP_011799158.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T23607 WP_003405820.1 NC_012207:c1233724-1233413 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T23607 NC_012207:c1233724-1233413 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT23607 WP_003405836.1 NC_012207:c1234044-1233724 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT23607 NC_012207:c1234044-1233724 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References