Detailed information of TA system TA236058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 709466..710016
• Replicon: chromosome
• Accession: NZ_CP092417
• Organism: Endozoicomonas sp. SCSIO W0465

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MJO57_RS03000
• Protein ID: WP_252022881.1
• Coordinates: 709466..709789 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MJO57_RS03005
• Protein ID: WP_252022884.1
• Coordinates: 709786..710016 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJO57_RS02980 (MJO57_02965)	704684..705277	-	594	WP_252022865.1	hypothetical protein	-
MJO57_RS02985	705330..705842	-	513	WP_252022876.1	PEP/pyruvate-binding domain-containing protein	-
MJO57_RS32650	705823..706707	-	885	WP_256493316.1	PEP/pyruvate-binding domain-containing protein	-
MJO57_RS02995 (MJO57_02975)	707139..709100	+	1962	WP_252022879.1	DNA mismatch repair endonuclease MutL	-
MJO57_RS03000 (MJO57_02980)	709466..709789	-	324	WP_252022881.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MJO57_RS03005 (MJO57_02985)	709786..710016	-	231	WP_252022884.1	antitoxin MazE family protein	Antitoxin
MJO57_RS03010 (MJO57_02990)	710294..710485	-	192	WP_252022885.1	hypothetical protein	-
MJO57_RS03015 (MJO57_02995)	710598..710840	-	243	WP_252022887.1	DUF6364 family protein	-
MJO57_RS03020 (MJO57_03000)	711056..711283	+	228	WP_252022889.1	hypothetical protein	-
MJO57_RS03025 (MJO57_03005)	711290..711574	+	285	WP_252022891.1	hypothetical protein	-
MJO57_RS03030 (MJO57_03010)	711737..712309	-	573	WP_252022893.1	TIGR04219 family outer membrane beta-barrel protein	-
MJO57_RS03035 (MJO57_03015)	712694..714358	-	1665	WP_252022895.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-101)

Antitoxin

• Antitoxin MazE-like (12-75)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11886.89 Da
• Isoelectric Point: 9.6242

>T236058 WP_252022881.1 NZ_CP092417:c709789-709466 [Endozoicomonas sp. SCSIO W0465]
MKRGDIVTVSIKGDYGKPRPALIIQSNLFPDHPSITILPITGELRDVPLFRYTLFESKDNGLNKTSQVMIDKITTIKADN
IGKTIGSLSQQELTEVTRLMALWLGMA

Nucleotide

• Length: 324 bp

>T236058 NZ_LR135243:221881-222147 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8861.87 Da
• Isoelectric Point: 5.1169

>AT236058 WP_252022884.1 NZ_CP092417:c710016-709786 [Endozoicomonas sp. SCSIO W0465]
MSTNASQPKTTRERVESHREALRQAGLRPLQIWVPDTRVKGFDLECQRQSAMLKSDKLERDTMDWIEQTSDDEGWA

Nucleotide

• Length: 231 bp

>AT236058 NZ_LR135243:221618-221881 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA