Detailed information of TA system TA236056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1229783..1230700
• Replicon: chromosome
• Accession: NZ_CP092412
• Organism: Bacillus velezensis strain HNU24

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MH925_RS06505
• Protein ID: WP_012117368.1
• Coordinates: 1229954..1230700 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MH925_RS06500
• Protein ID: WP_003154807.1
• Coordinates: 1229783..1229953 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MH925_RS06450	1224917..1225429	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
MH925_RS06455	1225542..1225775	+	234	WP_012117363.1	XtmA	-
MH925_RS06460	1225791..1226639	+	849	WP_012117364.1	hypothetical protein	-
MH925_RS06465	1226652..1227023	+	372	WP_012117365.1	XkdW family protein	-
MH925_RS06470	1227028..1227225	+	198	WP_012117366.1	XkdX family protein	-
MH925_RS06475	1227282..1228043	+	762	WP_012117367.1	hypothetical protein	-
MH925_RS06480	1228095..1228358	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MH925_RS06485	1228372..1228635	+	264	WP_003154813.1	phage holin	-
MH925_RS06490	1228649..1229527	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
MH925_RS06500	1229783..1229953	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MH925_RS06505	1229954..1230700	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MH925_RS06510	1230805..1231803	-	999	WP_007407255.1	inorganic phosphate transporter	-
MH925_RS06515	1231816..1232433	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MH925_RS06520	1232719..1234035	-	1317	WP_007610842.1	amino acid permease	-
MH925_RS06525	1234358..1235308	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T236056 WP_012117368.1 NZ_CP092412:c1230700-1229954 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T236056 NZ_LR135238:49593-49943 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
GCCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT236056 WP_003154807.1 NZ_CP092412:c1229953-1229783 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT236056 NZ_LR135238:49336-49599 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14