Detailed information of TA system TA236055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 493940..494577
• Replicon: chromosome
• Accession: NZ_CP092412
• Organism: Bacillus velezensis strain HNU24

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MH925_RS02550
• Protein ID: WP_003156187.1
• Coordinates: 494227..494577 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: MH925_RS02545
• Protein ID: WP_003156188.1
• Coordinates: 493940..494221 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MH925_RS02525	490305..490904	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
MH925_RS02530	490997..491362	+	366	WP_012116869.1	holo-ACP synthase	-
MH925_RS02535	491527..492534	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
MH925_RS02540	492651..493820	+	1170	WP_012116870.1	alanine racemase	-
MH925_RS02545	493940..494221	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MH925_RS02550	494227..494577	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MH925_RS02555	494695..495516	+	822	WP_003156182.1	STAS domain-containing protein	-
MH925_RS02560	495521..495886	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
MH925_RS02565	495889..496290	+	402	WP_003156178.1	anti-sigma regulatory factor	-
MH925_RS02570	496302..497309	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
MH925_RS02575	497373..497702	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
MH925_RS02580	497699..498181	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
MH925_RS02585	498147..498935	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
MH925_RS02590	498935..499537	+	603	WP_012116871.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T236055 WP_003156187.1 NZ_CP092412:494227-494577 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T236055 NZ_LR135237:c19722-19537 [Enterococcus faecium]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAA
TTTTGAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT236055 WP_003156188.1 NZ_CP092412:493940-494221 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT236055 NZ_LR135237:c19503-19072 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGGATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGTTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5