Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 165614..166524 Replicon plasmid Kp116-unnamed1
Accession NZ_CP092410
Organism Klebsiella pneumoniae strain IA-044

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag KZ664_RS26000 Protein ID WP_004144067.1
Coordinates 165614..166084 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag KZ664_RS26005 Protein ID WP_023287184.1
Coordinates 166081..166524 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ664_RS25975 (KZ664_26135) 161688..162656 - 969 WP_100117086.1 IS5 family transposase -
KZ664_RS25980 (KZ664_26140) 162647..162829 + 183 Protein_167 IS481 family transposase -
KZ664_RS25985 (KZ664_26145) 162833..163606 + 774 Protein_168 integrase core domain-containing protein -
KZ664_RS25990 (KZ664_26150) 164179..164439 + 261 WP_017901409.1 hypothetical protein -
KZ664_RS25995 (KZ664_26155) 164559..165527 - 969 WP_000654811.1 IS5 family transposase -
KZ664_RS26000 (KZ664_26160) 165614..166084 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
KZ664_RS26005 (KZ664_26165) 166081..166524 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
KZ664_RS26010 (KZ664_26170) 166625..167767 - 1143 Protein_173 translesion error-prone DNA polymerase V subunit UmuC -
KZ664_RS26015 (KZ664_26175) 167820..169838 - 2019 WP_257964656.1 ThiF family adenylyltransferase -
KZ664_RS26025 (KZ664_26185) 170620..170715 + 96 Protein_176 integrase core domain-containing protein -
KZ664_RS26030 (KZ664_26190) 170699..171330 + 632 Protein_177 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - cheD 1..175354 175354
- inside IScluster/Tn - - 161688..170368 8680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T236053 WP_004144067.1 NZ_CP092410:c166084-165614 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T236053 NZ_LR135236:17158-17508 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT236053 WP_023287184.1 NZ_CP092410:c166524-166081 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT236053 NZ_LR135236:16901-17164 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References