Detailed information of TA system TA236051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53668..54404
• Replicon: plasmid Kp93-unnamed1
• Accession: NZ_CP092409
• Organism: Klebsiella pneumoniae strain IA-021

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KZ660_RS25730
• Protein ID: WP_003026803.1
• Coordinates: 53922..54404 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KZ660_RS25725
• Protein ID: WP_003026799.1
• Coordinates: 53668..53934 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ660_RS25680 (KZ660_25810)	49730..50092	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
KZ660_RS25685 (KZ660_25815)	50142..50492	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
KZ660_RS25690 (KZ660_25820)	50850..51119	+	270	WP_004152102.1	hypothetical protein	-
KZ660_RS25695 (KZ660_25825)	51107..51682	+	576	WP_004152103.1	hypothetical protein	-
KZ660_RS25700 (KZ660_25830)	51713..52207	+	495	WP_004152104.1	DNA-binding protein	-
KZ660_RS25705 (KZ660_25835)	52251..52619	+	369	WP_004152105.1	hypothetical protein	-
KZ660_RS25710 (KZ660_25840)	52653..52856	+	204	WP_240267340.1	hemolysin expression modulator Hha	-
KZ660_RS25715 (KZ660_25845)	52905..53162	+	258	WP_004152107.1	hypothetical protein	-
KZ660_RS25720 (KZ660_25850)	53238..53492	+	255	WP_004152108.1	hypothetical protein	-
KZ660_RS25725 (KZ660_25855)	53668..53934	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KZ660_RS25730 (KZ660_25860)	53922..54404	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KZ660_RS25735 (KZ660_25865)	54583..55986	+	1404	WP_004113090.1	ISNCY family transposase	-
KZ660_RS25740 (KZ660_25870)	56017..56934	-	918	WP_158003452.1	S-4TM family putative pore-forming effector	-
KZ660_RS25745 (KZ660_25875)	56938..57936	-	999	WP_001610306.1	hypothetical protein	-
KZ660_RS25750 (KZ660_25880)	58140..59305	-	1166	Protein_60	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..169162	169162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T236051 WP_003026803.1 NZ_CP092409:53922-54404 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T236051 NZ_LR135235:183353-183619 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT236051 WP_003026799.1 NZ_CP092409:53668-53934 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT236051 NZ_LR135235:183090-183353 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66