Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13547..14190 Replicon plasmid Kp25-unnamed1
Accession NZ_CP092408
Organism Klebsiella quasipneumoniae strain MN-07

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag KZ661_RS25255 Protein ID WP_000754567.1
Coordinates 13774..14190 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag KZ661_RS25250 Protein ID WP_001261275.1
Coordinates 13547..13777 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ661_RS25245 (KZ661_25435) 10718..13294 + 2577 WP_017901322.1 nuclease domain-containing protein -
KZ661_RS25250 (KZ661_25440) 13547..13777 + 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZ661_RS25255 (KZ661_25445) 13774..14190 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ661_RS25260 (KZ661_25450) 14295..15836 - 1542 WP_240247760.1 IS66 family transposase -
KZ661_RS25265 (KZ661_25455) 15867..16217 - 351 WP_004189161.1 IS66 family insertion sequence element accessory protein TnpB -
KZ661_RS25270 (KZ661_25460) 16214..16654 - 441 WP_240247758.1 transposase -
KZ661_RS25275 (KZ661_25465) 16768..17996 + 1229 Protein_17 IS3 family transposase -
KZ661_RS25280 (KZ661_25470) 18447..19144 + 698 WP_240247761.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..223240 223240
- inside IScluster/Tn - - 14295..21932 7637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T236048 WP_000754567.1 NZ_CP092408:13774-14190 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T236048 NZ_LR135226:539219-539560 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT236048 WP_001261275.1 NZ_CP092408:13547-13777 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT236048 NZ_LR135226:538989-539219 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References