Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 179998..180908 Replicon plasmid Kp62-unnamed1
Accession NZ_CP092407
Organism Klebsiella pneumoniae strain CT-04

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag KZ663_RS26710 Protein ID WP_004144067.1
Coordinates 179998..180468 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag KZ663_RS26715 Protein ID WP_023287184.1
Coordinates 180465..180908 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ663_RS26690 (KZ663_26870) 175592..176803 + 1212 WP_017901247.1 M24 family metallopeptidase -
KZ663_RS26695 (KZ663_26875) 177142..178110 - 969 WP_167813129.1 IS5 family transposase -
KZ663_RS26700 (KZ663_26880) 178101..178430 + 330 Protein_184 IS481 family transposase -
KZ663_RS26705 (KZ663_26885) 178943..179911 - 969 WP_000654811.1 IS5 family transposase -
KZ663_RS26710 (KZ663_26890) 179998..180468 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
KZ663_RS26715 (KZ663_26895) 180465..180908 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
KZ663_RS26720 (KZ663_26900) 181009..182151 - 1143 Protein_188 translesion error-prone DNA polymerase V subunit UmuC -
KZ663_RS26725 (KZ663_26905) 182204..184222 - 2019 WP_257964656.1 ThiF family adenylyltransferase -
KZ663_RS26735 (KZ663_26915) 185004..185099 + 96 Protein_191 integrase core domain-containing protein -
KZ663_RS26740 (KZ663_26920) 185083..185714 + 632 Protein_192 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib cheD 1..189738 189738
- inside IScluster/Tn - - 177142..184752 7610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T236047 WP_004144067.1 NZ_CP092407:c180468-179998 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T236047 NZ_LR135226:218875-219141 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT236047 WP_023287184.1 NZ_CP092407:c180908-180465 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT236047 NZ_LR135226:218612-218875 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References