Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 33909..34819 Replicon plasmid Kp27-unnamed1
Accession NZ_CP092401
Organism Klebsiella pneumoniae strain NC-01

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KZ666_RS27700 Protein ID WP_004026354.1
Coordinates 34349..34819 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KZ666_RS27695 Protein ID WP_004181895.1
Coordinates 33909..34352 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ666_RS27675 (KZ666_27855) 29310..30017 + 708 WP_048268734.1 hypothetical protein -
KZ666_RS27680 (KZ666_27860) 30022..31053 + 1032 WP_170919122.1 CRISPR-associated protein Csf2 -
KZ666_RS27685 (KZ666_27865) 32115..32537 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KZ666_RS27690 (KZ666_27870) 32537..33808 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KZ666_RS27695 (KZ666_27875) 33909..34352 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KZ666_RS27700 (KZ666_27880) 34349..34819 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KZ666_RS27705 (KZ666_27885) 35274..35462 - 189 WP_024198076.1 hypothetical protein -
KZ666_RS27710 (KZ666_27890) 36075..36335 - 261 WP_032719456.1 hypothetical protein -
KZ666_RS27715 (KZ666_27895) 37058..37216 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
KZ666_RS27720 (KZ666_27900) 37819..38793 + 975 WP_064794367.1 hypothetical protein -
KZ666_RS27725 (KZ666_27905) 38991..39368 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..299847 299847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T236042 WP_004026354.1 NZ_CP092401:34349-34819 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T236042 NZ_LR135220:17182-17532 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT236042 WP_004181895.1 NZ_CP092401:33909-34352 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT236042 NZ_LR135220:16925-17188 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References