Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 189979..190622 Replicon plasmid Kp27-unnamed2
Accession NZ_CP092400
Organism Klebsiella pneumoniae strain NC-01

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag KZ666_RS27365 Protein ID WP_014386165.1
Coordinates 190206..190622 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag KZ666_RS27360 Protein ID WP_032408893.1
Coordinates 189979..190209 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ666_RS27335 (KZ666_27515) 185052..186035 - 984 WP_001471744.1 NAD(P)H-quinone oxidoreductase -
KZ666_RS27340 (KZ666_27520) 186054..187202 - 1149 WP_014386168.1 ABC transporter ATP-binding protein -
KZ666_RS27345 (KZ666_27525) 187373..188530 - 1158 WP_032408895.1 acetylornithine deacetylase -
KZ666_RS27350 (KZ666_27530) 188546..189220 - 675 WP_014386166.1 DUF1028 domain-containing protein -
KZ666_RS27355 (KZ666_27535) 189225..189659 - 435 WP_000103648.1 RidA family protein -
KZ666_RS27360 (KZ666_27540) 189979..190209 + 231 WP_032408893.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZ666_RS27365 (KZ666_27545) 190206..190622 + 417 WP_014386165.1 PIN domain-containing protein Toxin
KZ666_RS27370 (KZ666_27550) 190945..191913 + 969 WP_016809156.1 IS5-like element IS903B family transposase -
KZ666_RS27375 (KZ666_27555) 192093..192467 - 375 WP_032408891.1 hypothetical protein -
KZ666_RS27380 (KZ666_27560) 192523..192849 - 327 WP_004152639.1 hypothetical protein -
KZ666_RS27385 (KZ666_27565) 192846..193574 - 729 WP_046624007.1 plasmid SOS inhibition protein A -
KZ666_RS27390 (KZ666_27570) 193571..194002 - 432 WP_261949546.1 conjugation system SOS inhibitor PsiB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..210837 210837
- flank IS/Tn - - 190945..191913 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-130)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15127.44 Da        Isoelectric Point: 7.8841

>T236041 WP_014386165.1 NZ_CP092400:190206-190622 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Download         Length: 417 bp

>T236041 NZ_LR135219:476839-477180 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8854.89 Da        Isoelectric Point: 4.5112

>AT236041 WP_032408893.1 NZ_CP092400:189979-190209 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Download         Length: 231 bp

>AT236041 NZ_LR135219:476609-476839 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References