Detailed information of TA system TA023604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016873..1017492
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JTY_RS04815
• Protein ID: WP_003404726.1
• Coordinates: 1017058..1017492 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: JTY_RS04810
• Protein ID: WP_003404724.1
• Coordinates: 1016873..1017052 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS04800	1012346..1013926	+	1581	WP_011799139.1	serine hydrolase	-
JTY_RS04805	1013923..1016316	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JTY_RS22350	1016589..1016747	+	159	WP_003404720.1	hypothetical protein	-
JTY_RS04810	1016873..1017052	+	180	WP_003404724.1	antitoxin	Antitoxin
JTY_RS04815	1017058..1017492	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JTY_RS04820	1017590..1018363	+	774	WP_003404735.1	VOC family protein	-
JTY_RS04825	1018428..1018877	+	450	WP_003404738.1	hypothetical protein	-
JTY_RS04830	1019012..1019242	-	231	WP_003898642.1	hypothetical protein	-
JTY_RS04835	1019409..1020917	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
JTY_RS04840	1020919..1022157	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T23604 WP_003404726.1 NC_012207:1017058-1017492 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T23604 NC_012207:1017058-1017492 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT23604 WP_003404724.1 NC_012207:1016873-1017052 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT23604 NC_012207:1016873-1017052 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5