Detailed information of TA system TA236039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 56026..56762
• Replicon: plasmid Kp27-unnamed2
• Accession: NZ_CP092400
• Organism: Klebsiella pneumoniae strain NC-01

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: KZ666_RS26610
• Protein ID: WP_004187044.1
• Coordinates: 56280..56762 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KZ666_RS26605
• Protein ID: WP_003026799.1
• Coordinates: 56026..56292 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ666_RS26590 (KZ666_26770)	51498..52892	+	1395	WP_032439652.1	cytosine permease	-
KZ666_RS26595 (KZ666_26775)	52904..54112	+	1209	WP_032439650.1	imidazolonepropionase	-
KZ666_RS26600 (KZ666_26780)	54105..54914	+	810	WP_023329017.1	N-formylglutamate deformylase	-
KZ666_RS26605 (KZ666_26785)	56026..56292	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KZ666_RS26610 (KZ666_26790)	56280..56762	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
KZ666_RS26615 (KZ666_26795)	56969..58303	+	1335	WP_077257889.1	ISNCY family transposase	-
KZ666_RS26620 (KZ666_26800)	58352..58750	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
KZ666_RS26625 (KZ666_26805)	58898..59008	-	111	Protein_58	IS3 family transposase	-
KZ666_RS26630 (KZ666_26810)	59283..60219	+	937	Protein_59	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..210837	210837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T236039 WP_004187044.1 NZ_CP092400:56280-56762 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T236039 NZ_LR135207:22756-23106 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT236039 WP_003026799.1 NZ_CP092400:56026-56292 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT236039 NZ_LR135207:22499-22762 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66