Detailed information of TA system TA236038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 11119..11762
• Replicon: plasmid Kp27-unnamed2
• Accession: NZ_CP092400
• Organism: Klebsiella pneumoniae strain NC-01

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KZ666_RS26390
• Protein ID: WP_016236302.1
• Coordinates: 11346..11762 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: KZ666_RS26385
• Protein ID: WP_001261282.1
• Coordinates: 11119..11349 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ666_RS26360 (KZ666_26540)	6835..7092	-	258	WP_023292103.1	hypothetical protein	-
KZ666_RS26365 (KZ666_26545)	7570..8298	+	729	Protein_6	CSS-motif domain-containing protein	-
KZ666_RS26370 (KZ666_26550)	8314..9150	+	837	WP_032439674.1	EAL domain-containing protein	-
KZ666_RS26375 (KZ666_26555)	9768..10199	-	432	WP_174805835.1	hypothetical protein	-
KZ666_RS26380 (KZ666_26560)	10740..11162	-	423	WP_046624301.1	hypothetical protein	-
KZ666_RS26385 (KZ666_26565)	11119..11349	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KZ666_RS26390 (KZ666_26570)	11346..11762	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZ666_RS26395 (KZ666_26575)	11836..13398	+	1563	WP_004206609.1	AAA family ATPase	-
KZ666_RS26400 (KZ666_26580)	13383..14405	+	1023	WP_032439672.1	helicase UvrD	-
KZ666_RS26405 (KZ666_26585)	14950..15858	+	909	WP_032439686.1	HNH endonuclease	-
KZ666_RS26410 (KZ666_26590)	16044..16394	-	351	WP_046624298.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..210837	210837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T236038 WP_016236302.1 NZ_CP092400:11346-11762 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T236038 NZ_LR135206:c3040-2690 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGGGTTCTGTTGCAAAGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT236038 WP_001261282.1 NZ_CP092400:11119-11349 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT236038 NZ_LR135206:c3314-3042 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3