236037

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 257953..258689 Replicon plasmid Kp23-unnamed1
Accession NZ_CP092398
Organism Klebsiella pneumoniae strain PA-01

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KZ665_RS27040 Protein ID WP_003026803.1
Coordinates 257953..258435 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KZ665_RS27045 Protein ID WP_003026799.1
Coordinates 258423..258689 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ665_RS27015 253519..254169 + 651 WP_112029135.1 GntR family transcriptional regulator -
KZ665_RS27020 254284..254646 + 363 Protein_259 ATP-binding protein -
KZ665_RS27025 254738..255166 - 429 WP_064169130.1 Rrf2 family transcriptional regulator -
KZ665_RS27030 255302..256189 + 888 Protein_261 NAD(P)/FAD-dependent oxidoreductase -
KZ665_RS27035 256374..257794 + 1421 Protein_262 IS3 family transposase -
KZ665_RS27040 257953..258435 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KZ665_RS27045 258423..258689 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KZ665_RS27050 259499..260634 + 1136 WP_202394502.1 IS3 family transposase -
KZ665_RS27055 260855..262585 - 1731 Protein_266 AAA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..307227 307227
- inside IScluster/Tn - - 256374..270781 14407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T236037 WP_003026803.1 NZ_CP092398:c258435-257953 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T236037 NZ_LR135204:95483-95878 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT236037 WP_003026799.1 NZ_CP092398:c258689-258423 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT236037 NZ_LR135204:95209-95481 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References