Detailed information of TA system TA236036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 245369..246279
• Replicon: plasmid Kp23-unnamed1
• Accession: NZ_CP092398
• Organism: Klebsiella pneumoniae strain PA-01

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KZ665_RS26980
• Protein ID: WP_112029140.1
• Coordinates: 245809..246279 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W9B1L8
• Locus tag: KZ665_RS26975
• Protein ID: WP_016528982.1
• Coordinates: 245369..245812 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ665_RS26945	240864..241061	+	198	Protein_244	hypothetical protein	-
KZ665_RS26950	241064..241609	-	546	WP_112029143.1	winged helix DNA-binding protein	-
KZ665_RS26955	241808..242530	+	723	WP_086523242.1	DUF969 domain-containing protein	-
KZ665_RS26960	242527..243513	+	987	WP_112029142.1	DUF979 domain-containing protein	-
KZ665_RS26965	243523..244518	+	996	WP_112029141.1	DUF2891 domain-containing protein	-
KZ665_RS26970	244679..245312	+	634	Protein_249	DUF4113 domain-containing protein	-
KZ665_RS26975	245369..245812	+	444	WP_016528982.1	DUF2384 domain-containing protein	Antitoxin
KZ665_RS26980	245809..246279	+	471	WP_112029140.1	RES family NAD+ phosphorylase	Toxin
KZ665_RS26985	246389..246649	-	261	WP_112029139.1	hypothetical protein	-
KZ665_RS26990	247008..248753	-	1746	WP_112029138.1	biotin carboxylase N-terminal domain-containing protein	-
KZ665_RS26995	248753..250336	-	1584	WP_261945505.1	urea amidolyase family protein	-
KZ665_RS27000	250349..251155	-	807	WP_112029136.1	putative hydro-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..307227	307227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17591.95 Da
• Isoelectric Point: 4.7424

>T236036 WP_112029140.1 NZ_CP092398:245809-246279 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSINVPDELIQSANMD
ELPVNWADEDAPRELADYGDAWSFTRTSVALRVPSALSPVEFNYLLNPEHSEFYGIVQMAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T236036 NZ_LR135204:55513-55788 [Enterococcus faecium]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT236036 WP_016528982.1 NZ_CP092398:245369-245812 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEIIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT236036 NZ_LR135204:55254-55520 [Enterococcus faecium]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Antitoxin

• AlphaFold DB: W9B1L8