Detailed information of TA system TA236035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/GNAT-DUF1778
• Location: 187795..188546
• Replicon: plasmid Kp23-unnamed1
• Accession: NZ_CP092398
• Organism: Klebsiella pneumoniae strain PA-01

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A8T3UHS1
• Locus tag: KZ665_RS26650
• Protein ID: WP_004883482.1
• Coordinates: 188064..188546 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A8T3UKU4
• Locus tag: KZ665_RS26645
• Protein ID: WP_004883484.1
• Coordinates: 187795..188073 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ665_RS26625	182951..184912	+	1962	WP_112029085.1	sigma 54-interacting transcriptional regulator	-
KZ665_RS26630	185088..185492	+	405	WP_086528925.1	DUF2251 domain-containing protein	-
KZ665_RS26635	185827..186660	+	834	WP_004883492.1	GIY-YIG nuclease family protein	-
KZ665_RS26640	186765..186989	+	225	WP_226879455.1	bacteriocin immunity protein	-
KZ665_RS26645	187795..188073	+	279	WP_004883484.1	DUF1778 domain-containing protein	Antitoxin
KZ665_RS26650	188064..188546	+	483	WP_004883482.1	GNAT family N-acetyltransferase	Toxin
KZ665_RS26655	188599..189081	-	483	WP_004196929.1	hypothetical protein	-
KZ665_RS26660	189460..190959	-	1500	WP_086528915.1	lipopolysaccharide kinase InaA family protein	-
KZ665_RS26665	190987..192720	-	1734	WP_032412835.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..307227	307227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (69-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17491.36 Da
• Isoelectric Point: 9.4950

>T236035 WP_004883482.1 NZ_CP092398:188064-188546 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIAEFCCQDQVINEWLKKKALRNHGTGISRVFVVCSENSKRVIAYYCLSSGSVHRNTVTGTYRRNAP
AAIPVIVLGRLAVDAAWAGKGIGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVVAFYTKFGFETSAVNALTLLFPVKV

Nucleotide

• Length: 483 bp

>T236035 NZ_LR135204:44675-44860 [Enterococcus faecium]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCAAAAAA
TTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10720.23 Da
• Isoelectric Point: 4.5423

>AT236035 WP_004883484.1 NZ_CP092398:187795-188073 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFLLDDKQFDTFIEELDAPVTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT236035 NZ_LR135204:44894-45325 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG