Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 135232..136142 Replicon plasmid Kp4-unnamed1
Accession NZ_CP092395
Organism Klebsiella pneumoniae strain MN-03

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag KZ659_RS26710 Protein ID WP_004144067.1
Coordinates 135232..135702 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag KZ659_RS26715 Protein ID WP_023287184.1
Coordinates 135699..136142 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ659_RS26690 (KZ659_26955) 130613..131461 - 849 WP_004118155.1 transporter substrate-binding domain-containing protein -
KZ659_RS26695 (KZ659_26960) 131618..132571 + 954 WP_020481021.1 LysR substrate-binding domain-containing protein -
KZ659_RS26700 (KZ659_26965) 132658..133869 + 1212 WP_017901247.1 M24 family metallopeptidase -
KZ659_RS26705 (KZ659_26970) 134177..135145 - 969 WP_000654811.1 IS5 family transposase -
KZ659_RS26710 (KZ659_26975) 135232..135702 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
KZ659_RS26715 (KZ659_26980) 135699..136142 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
KZ659_RS26720 (KZ659_26985) 136243..137385 - 1143 Protein_142 translesion error-prone DNA polymerase V subunit UmuC -
KZ659_RS26725 (KZ659_26990) 137438..138619 - 1182 WP_227550492.1 ThiF family adenylyltransferase -
KZ659_RS26730 (KZ659_26995) 139157..139354 - 198 Protein_144 hypothetical protein -
KZ659_RS26740 (KZ659_27005) 140133..140228 + 96 Protein_146 transposase -
KZ659_RS26745 (KZ659_27010) 140212..140843 + 632 Protein_147 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..144867 144867
- inside IScluster/Tn - - 134177..140075 5898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T236033 WP_004144067.1 NZ_CP092395:c135702-135232 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T236033 NZ_LR135203:218875-219141 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT236033 WP_023287184.1 NZ_CP092395:c136142-135699 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT236033 NZ_LR135203:218612-218875 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References