Detailed information of TA system TA236032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 43427..43952
• Replicon: plasmid Kp4-unnamed2
• Accession: NZ_CP092394
• Organism: Klebsiella pneumoniae strain MN-03

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: KZ659_RS25850
• Protein ID: WP_016946795.1
• Coordinates: 43647..43952 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: KZ659_RS25845
• Protein ID: WP_004197642.1
• Coordinates: 43427..43645 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ659_RS25810 (KZ659_26075)	38562..39242	-	681	WP_261949192.1	transporter substrate-binding domain-containing protein	-
KZ659_RS25815 (KZ659_26080)	39331..40125	-	795	WP_023280950.1	IclR family transcriptional regulator	-
KZ659_RS25820 (KZ659_26085)	40509..40904	+	396	WP_071838318.1	hypothetical protein	-
KZ659_RS25825 (KZ659_26090)	40909..41070	-	162	Protein_45	IS110 family transposase	-
KZ659_RS25830 (KZ659_26095)	41127..42095	-	969	WP_000654805.1	IS5-like element IS903B family transposase	-
KZ659_RS25835 (KZ659_26100)	42177..42593	-	417	WP_126493404.1	type II toxin-antitoxin system VapC family toxin	-
KZ659_RS25840 (KZ659_26105)	42590..42820	-	231	WP_126493402.1	type II toxin-antitoxin system VapB family antitoxin	-
KZ659_RS25845 (KZ659_26110)	43427..43645	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KZ659_RS25850 (KZ659_26115)	43647..43952	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
KZ659_RS25855 (KZ659_26120)	44110..44793	+	684	WP_023280892.1	hypothetical protein	-
KZ659_RS25860 (KZ659_26125)	44796..45572	+	777	WP_049245386.1	site-specific integrase	-
KZ659_RS25865 (KZ659_26130)	45630..45887	-	258	WP_000764642.1	hypothetical protein	-
KZ659_RS25870 (KZ659_26135)	46016..46120	-	105	WP_032409716.1	hypothetical protein	-
KZ659_RS25875 (KZ659_26140)	46655..47521	+	867	WP_004118283.1	replication initiation protein	-
KZ659_RS25880 (KZ659_26145)	47878..48147	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76239	76239
	-	flank	IS/Tn	-	-	41127..42050	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T236032 WP_016946795.1 NZ_CP092394:43647-43952 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T236032 NZ_LR135199:45550-45900 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT236032 WP_004197642.1 NZ_CP092394:43427-43645 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT236032 NZ_LR135199:45293-45556 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA