Detailed information of TA system TA236030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200624..201260
• Replicon: chromosome
• Accession: NZ_CP092387
• Organism: Priestia megaterium strain BP01R2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: MGJ28_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 200910..201260 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: MGJ28_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 200624..200905 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MGJ28_RS01105 (MGJ28_01105)	195990..196961	+	972	WP_241096699.1	UV DNA damage repair endonuclease UvsE	-
MGJ28_RS01110 (MGJ28_01110)	196970..197572	-	603	WP_013081417.1	rhomboid family intramembrane serine protease	-
MGJ28_RS01115 (MGJ28_01115)	197637..198002	+	366	WP_025753519.1	holo-ACP synthase	-
MGJ28_RS01120 (MGJ28_01120)	198061..199119	+	1059	WP_241096700.1	outer membrane lipoprotein carrier protein LolA	-
MGJ28_RS01125 (MGJ28_01125)	199233..200423	+	1191	WP_034654856.1	alanine racemase	-
MGJ28_RS01130 (MGJ28_01130)	200624..200905	+	282	WP_013055003.1	hypothetical protein	Antitoxin
MGJ28_RS01135 (MGJ28_01135)	200910..201260	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MGJ28_RS01140 (MGJ28_01140)	201420..202256	+	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
MGJ28_RS01145 (MGJ28_01145)	202259..202615	+	357	WP_013055006.1	STAS domain-containing protein	-
MGJ28_RS01150 (MGJ28_01150)	202619..203020	+	402	WP_013055007.1	anti-sigma regulatory factor	-
MGJ28_RS01155 (MGJ28_01155)	203034..204044	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
MGJ28_RS01160 (MGJ28_01160)	204104..204436	+	333	WP_013055009.1	anti-sigma factor antagonist	-
MGJ28_RS01165 (MGJ28_01165)	204433..204918	+	486	WP_016762758.1	anti-sigma B factor RsbW	-
MGJ28_RS01170 (MGJ28_01170)	204884..205678	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T236030 WP_013055004.1 NZ_CP092387:200910-201260 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T236030 NZ_LR135197:220398-220664 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT236030 WP_013055003.1 NZ_CP092387:200624-200905 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT236030 NZ_LR135197:220135-220398 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9