236029

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1358735..1359652 Replicon chromosome
Accession NZ_CP092383
Organism Bacillus velezensis strain SF327

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag MID02_RS06955 Protein ID WP_003154806.1
Coordinates 1358906..1359652 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag MID02_RS06950 Protein ID WP_003154807.1
Coordinates 1358735..1358905 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MID02_RS06910 (MID02_06910) 1353966..1355588 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
MID02_RS06915 (MID02_06915) 1355601..1355972 + 372 WP_014304856.1 XkdW family protein -
MID02_RS06920 (MID02_06920) 1355978..1356175 + 198 WP_003154819.1 XkdX family protein -
MID02_RS06925 (MID02_06925) 1356232..1356993 + 762 WP_024085194.1 hypothetical protein -
MID02_RS06930 (MID02_06930) 1357045..1357308 + 264 WP_003154815.1 hemolysin XhlA family protein -
MID02_RS06935 (MID02_06935) 1357322..1357585 + 264 WP_239819480.1 phage holin -
MID02_RS06940 (MID02_06940) 1357599..1358477 + 879 WP_014304858.1 N-acetylmuramoyl-L-alanine amidase -
MID02_RS06945 (MID02_06945) 1358513..1358638 - 126 WP_003154809.1 hypothetical protein -
MID02_RS06950 (MID02_06950) 1358735..1358905 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
MID02_RS06955 (MID02_06955) 1358906..1359652 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
MID02_RS06960 (MID02_06960) 1359757..1360755 - 999 WP_003154805.1 inorganic phosphate transporter -
MID02_RS06965 (MID02_06965) 1360768..1361385 - 618 WP_003154804.1 DUF47 domain-containing protein -
MID02_RS06970 (MID02_06970) 1361671..1362987 - 1317 WP_003154801.1 amino acid permease -
MID02_RS06975 (MID02_06975) 1363311..1364261 + 951 WP_069013314.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T236029 WP_003154806.1 NZ_CP092383:c1359652-1358906 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T236029 NZ_LR135193:49943-50128 [Enterococcus faecium]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCAAAAAA
TTTTAAAGGATGCGGGATTGAAATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT236029 WP_003154807.1 NZ_CP092383:c1358905-1358735 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT236029 NZ_LR135193:50162-50593 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References