Detailed information of TA system TA236028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 471083..471720
• Replicon: chromosome
• Accession: NZ_CP092383
• Organism: Bacillus velezensis strain SF327

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MID02_RS02435
• Protein ID: WP_003156187.1
• Coordinates: 471370..471720 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: MID02_RS02430
• Protein ID: WP_003156188.1
• Coordinates: 471083..471364 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MID02_RS02410 (MID02_02410)	467449..468048	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
MID02_RS02415 (MID02_02415)	468141..468506	+	366	WP_014304402.1	holo-ACP synthase	-
MID02_RS02420 (MID02_02420)	468670..469677	+	1008	WP_151295737.1	outer membrane lipoprotein carrier protein LolA	-
MID02_RS02425 (MID02_02425)	469794..470963	+	1170	WP_239819360.1	alanine racemase	-
MID02_RS02430 (MID02_02430)	471083..471364	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MID02_RS02435 (MID02_02435)	471370..471720	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MID02_RS02440 (MID02_02440)	471838..472659	+	822	WP_014304404.1	STAS domain-containing protein	-
MID02_RS02445 (MID02_02445)	472664..473029	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
MID02_RS02450 (MID02_02450)	473032..473433	+	402	WP_003156178.1	anti-sigma regulatory factor	-
MID02_RS02455 (MID02_02455)	473445..474452	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
MID02_RS02460 (MID02_02460)	474516..474845	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
MID02_RS02465 (MID02_02465)	474842..475324	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
MID02_RS02470 (MID02_02470)	475290..476078	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
MID02_RS02475 (MID02_02475)	476078..476680	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T236028 WP_003156187.1 NZ_CP092383:471370-471720 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T236028 NZ_LR135192:c131173-130901 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT236028 WP_003156188.1 NZ_CP092383:471083-471364 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT236028 NZ_LR135192:c131436-131173 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5