Detailed information of TA system TA236014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 884651..885396
• Replicon: chromosome
• Accession: NZ_CP092380
• Organism: Xanthomonas oryzae strain GD1358

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: MJJ09_RS04280
• Protein ID: WP_199285672.1
• Coordinates: 885067..885396 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: MJJ09_RS04275
• Protein ID: WP_011407754.1
• Coordinates: 884651..885070 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ09_RS04255 (MJJ09_04250)	880943..881269	-	327	Protein_800	hypothetical protein	-
MJJ09_RS04260 (MJJ09_04255)	881571..882805	+	1235	Protein_801	ISL3 family transposase	-
MJJ09_RS04265 (MJJ09_04260)	882843..883481	-	639	WP_011407753.1	phosphate phosphatase	-
MJJ09_RS04270 (MJJ09_04265)	883634..884599	-	966	Protein_803	IS5-like element ISXo1 family transposase	-
MJJ09_RS04275 (MJJ09_04270)	884651..885070	-	420	WP_011407754.1	macro domain-containing protein	Antitoxin
MJJ09_RS04280 (MJJ09_04275)	885067..885396	-	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
MJJ09_RS04285 (MJJ09_04280)	885499..886816	+	1318	Protein_806	IS701-like element ISXo15 family transposase	-
MJJ09_RS04290 (MJJ09_04285)	886860..887195	-	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
MJJ09_RS04295 (MJJ09_04290)	887527..888453	-	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
MJJ09_RS04300 (MJJ09_04295)	888499..889290	+	792	WP_011407758.1	polysaccharide deacetylase family protein	-
MJJ09_RS04310 (MJJ09_04305)	889592..890263	-	672	WP_011407759.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	881571..886816	5245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T236014 WP_199285672.1 NZ_CP092380:c885396-885067 [Xanthomonas oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T236014 NZ_LR135170:430167-430508 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT236014 WP_011407754.1 NZ_CP092380:c885070-884651 [Xanthomonas oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT236014 NZ_LR135170:429937-430167 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG