Detailed information of TA system TA236010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3015071..3015816
• Replicon: chromosome
• Accession: NZ_CP092379
• Organism: Xanthomonas oryzae strain GIV

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: MJJ06_RS14250
• Protein ID: WP_199285672.1
• Coordinates: 3015071..3015400 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: MJJ06_RS14255
• Protein ID: WP_011407754.1
• Coordinates: 3015397..3015816 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ06_RS14220 (MJJ06_14210)	3010202..3010873	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
MJJ06_RS14230 (MJJ06_14220)	3011175..3011966	-	792	WP_011407758.1	polysaccharide deacetylase family protein	-
MJJ06_RS14235 (MJJ06_14225)	3012012..3012938	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
MJJ06_RS14240 (MJJ06_14230)	3013270..3013605	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
MJJ06_RS14245 (MJJ06_14235)	3013649..3014968	-	1320	WP_011407756.1	IS701 family transposase	-
MJJ06_RS14250 (MJJ06_14240)	3015071..3015400	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
MJJ06_RS14255 (MJJ06_14245)	3015397..3015816	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
MJJ06_RS14260 (MJJ06_14250)	3015868..3016836	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
MJJ06_RS14265 (MJJ06_14255)	3016989..3017627	+	639	WP_011407753.1	phosphate phosphatase	-
MJJ06_RS14270 (MJJ06_14260)	3017665..3018900	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
MJJ06_RS14275 (MJJ06_14265)	3019202..3019528	+	327	Protein_2735	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3013649..3018900	5251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T236010 WP_199285672.1 NZ_CP092379:3015071-3015400 [Xanthomonas oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T236010 NZ_LR135168:c163645-163199 [Mycobacterium ulcerans]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGTGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGATCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCAGCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGCCGGCTGCCGGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT236010 WP_011407754.1 NZ_CP092379:3015397-3015816 [Xanthomonas oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT236010 NZ_LR135168:c163838-163650 [Mycobacterium ulcerans]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAAGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA