236007

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 12276..13413 Replicon plasmid pK21EFM001_1_4
Accession NZ_CP092375
Organism Enterococcus faecium strain V13-21-E11-012-001

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag LRL10_RS15140 Protein ID WP_001284311.1
Coordinates 12276..13139 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LRL10_RS15145 Protein ID WP_000301765.1
Coordinates 13141..13413 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRL10_RS15115 (LRL10_15895) 8154..9143 - 990 Protein_12 ISL3-like element ISEfa11 family transposase -
LRL10_RS15120 (LRL10_15900) 9388..9639 + 252 Protein_13 GNAT family N-acetyltransferase -
LRL10_RS15125 (LRL10_15905) 9732..10526 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
LRL10_RS15490 10936..11019 + 84 Protein_15 MLS leader peptide -
LRL10_RS15130 (LRL10_15910) 11068..11151 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
LRL10_RS15135 (LRL10_15915) 11276..12013 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LRL10_RS15140 (LRL10_15920) 12276..13139 - 864 WP_001284311.1 zeta toxin family protein Toxin
LRL10_RS15145 (LRL10_15925) 13141..13413 - 273 WP_000301765.1 antitoxin Antitoxin
LRL10_RS15150 (LRL10_15930) 13430..13645 - 216 WP_001835296.1 peptide-binding protein -
LRL10_RS15155 (LRL10_15935) 13737..13994 - 258 Protein_21 chromosome partitioning protein ParA -
LRL10_RS15160 (LRL10_15940) 14050..14730 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
LRL10_RS15165 (LRL10_15945) 14841..15986 + 1146 WP_232407632.1 ISL3 family transposase -
LRL10_RS15495 16036..16146 - 111 WP_277989191.1 Rep family protein -
LRL10_RS15170 (LRL10_15950) 16213..16368 - 156 WP_002299939.1 CopG family transcriptional regulator -
LRL10_RS15175 (LRL10_15955) 16978..17199 + 222 WP_002299940.1 hypothetical protein -
LRL10_RS15180 (LRL10_15960) 17200..17457 + 258 WP_002299941.1 hypothetical protein -
LRL10_RS15185 (LRL10_15965) 17593..17877 - 285 WP_079200807.1 Rep family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3')-III / erm(B) / VanHAX - 1..44886 44886
- inside IScluster/Tn aph(3')-III / erm(B) / VanHAX - 714..43131 42417


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T236007 WP_001284311.1 NZ_CP092375:c13139-12276 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T236007 NZ_LR134536:c1953265-1953056 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT236007 WP_000301765.1 NZ_CP092375:c13413-13141 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT236007 NZ_LR134536:c1953565-1953272 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References