Detailed information of TA system TA235990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 16597..17605
• Replicon: plasmid unnamed1
• Accession: NZ_CP092371
• Organism: Flavobacterium sp. CBA20B-1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MG290_RS14450
• Protein ID: WP_264563261.1
• Coordinates: 16597..17160 (-)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: MG290_RS14455
• Protein ID: WP_264563260.1
• Coordinates: 17150..17605 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MG290_RS14430 (MG290_14415)	12251..13429	-	1179	WP_264563265.1	restriction endonuclease subunit S	-
MG290_RS14435 (MG290_14420)	13426..14973	-	1548	WP_264563264.1	type I restriction-modification system subunit M	-
MG290_RS14440 (MG290_14425)	14980..15663	-	684	WP_264563263.1	hypothetical protein	-
MG290_RS14445 (MG290_14430)	15905..16471	-	567	WP_264563262.1	recombinase family protein	-
MG290_RS14450 (MG290_14435)	16597..17160	-	564	WP_264563261.1	PIN domain-containing protein	Toxin
MG290_RS14455 (MG290_14440)	17150..17605	-	456	WP_264563260.1	helix-turn-helix domain-containing protein	Antitoxin
MG290_RS14460 (MG290_14445)	17720..18175	-	456	WP_264563259.1	hypothetical protein	-
MG290_RS14465 (MG290_14450)	18221..19474	+	1254	WP_264563258.1	Y-family DNA polymerase	-
MG290_RS14470 (MG290_14455)	19471..19938	+	468	WP_264563257.1	S24 family peptidase	-
MG290_RS14475 (MG290_14460)	20020..20196	-	177	WP_264563256.1	histone H1	-
MG290_RS14480 (MG290_14465)	20432..21229	-	798	WP_264563255.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64592	64592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (81-127)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 21414.74 Da
• Isoelectric Point: 5.2668

>T235990 WP_264563261.1 NZ_CP092371:c17160-16597 [Flavobacterium sp. CBA20B-1]
MIHSVRYKAVLDTNVIFPIVIRDLLFWFAHYDLYTPKWSKIIFDEWKSVMIRKGVESSQAEKRINIANLAFPDALVLNYE
GLIDTLSLPDKDDRHVLAAAIKVNANIIVTNNLKDFPDDYLDSFGLKAVCADDFLTDIIDLNPKIALQAFKDLVLNRKNP
AMDEFQVLESFRKNGLNQTADYLHALL

Nucleotide

• Length: 564 bp

>T235990 NZ_LR134529:1974683-1975009 [Bartonella vinsonii]
ATGAGGCGTGGTGACATCTATATGGTAGATTTGGAACCAATTCAAGGACGAGAACAGCGTGGGTATCGTCCTGTCGTCAT
TGTTTCTCCTGATGATTTTAATCAAGCAACAGGTTTACCCGTTATTTTGCCGATCACAAGCGGTGGAAATTTTGCACGTC
GTATTGGATTTGCGGTGCCGCTTATGGGTACAAGAACAAGGGGTGTCATTCGTTGCGATCAACCCCGTGTTCTCGATTTG
GTGGTGCGCAATGGGCGTAAAGTAGAAAGTTTGCCAACAGCTATTATGGATGAGGTATTGGCGAAGGTTGTTACGATCTT
TAGTTAA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 17407.20 Da
• Isoelectric Point: 6.2593

>AT235990 WP_264563260.1 NZ_CP092371:c17605-17150 [Flavobacterium sp. CBA20B-1]
MEALDYIKRPTKKEQRVAIESYSALSSILSQLKKDDTEIEIEETNDRIKIPFKALKLLSEILKSMSEGNPISIVPIATEV
TTQKAAEILGCSRPHLVKLLEQGEIDFTMVGRHRRILFEDVIKYKNEMKQKQKKHIIDIMNFDDEIGLYDS

Nucleotide

• Length: 456 bp

>AT235990 NZ_LR134529:1974429-1974686 [Bartonella vinsonii]
ATGCACACAACAAAATTACGTAAAGTTGGAGGGTCAGTTATGCTCTCTATACCGCCCGCATTGCTTGATGTTCTTCATCT
TTCTGAAAACACGCAAGTTGGTTTGGCTATTGATAATGGCCGTCTTGTTGTTGAACCATCAGTTCAGTCGCGCTACACGA
TGGCTGAGTTATTAGCTGCTTCCGATTATTCGCAGCCACAATCACCTGAAGACCGTGAGTGGGTTGATGCCTCTGCTGTT
GGGGGCGAGCTTATATGA