Detailed information of TA system TA023598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 719272..719936
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P96917
• Locus tag: JTY_RS03290
• Protein ID: WP_003403246.1
• Coordinates: 719529..719936 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WF18
• Locus tag: JTY_RS03285
• Protein ID: WP_003403244.1
• Coordinates: 719272..719532 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS03260	715449..716606	+	1158	WP_003903091.1	hypothetical protein	-
JTY_RS03265	716617..717564	+	948	WP_003403232.1	DUF732 domain-containing protein	-
JTY_RS03270	717657..717911	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	-
JTY_RS03275	717911..718306	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	-
JTY_RS03280	718400..719140	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
JTY_RS03285	719272..719532	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JTY_RS03290	719529..719936	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	Toxin
JTY_RS03295	720008..721159	-	1152	WP_003403248.1	FIST C-terminal domain-containing protein	-
JTY_RS03300	721252..722979	-	1728	WP_010950423.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-57)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14376.37 Da
• Isoelectric Point: 4.3568

>T23598 WP_003403246.1 NC_012207:719529-719936 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Nucleotide

• Length: 408 bp

>T23598 NC_012207:719529-719936 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9492.84 Da
• Isoelectric Point: 11.9108

>AT23598 WP_003403244.1 NC_012207:719272-719532 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Nucleotide

• Length: 261 bp

>AT23598 NC_012207:719272-719532 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• PDB: 3DBO

Antitoxin

• AlphaFold DB: A0A7U4BSE4