Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 509868..510504 | Replicon | chromosome |
| Accession | NZ_CP092369 | ||
| Organism | Bacillus subtilis strain ZW | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | MID01_RS02600 | Protein ID | WP_003156187.1 |
| Coordinates | 510154..510504 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | MID01_RS02595 | Protein ID | WP_003225183.1 |
| Coordinates | 509868..510149 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MID01_RS02575 (506228) | 506228..506827 | - | 600 | WP_041850724.1 | rhomboid family intramembrane serine protease | - |
| MID01_RS02580 (506922) | 506922..507287 | + | 366 | WP_015252768.1 | holo-ACP synthase | - |
| MID01_RS02585 (507453) | 507453..508469 | + | 1017 | WP_072557167.1 | outer membrane lipoprotein carrier protein LolA | - |
| MID01_RS02590 (508583) | 508583..509752 | + | 1170 | WP_015252766.1 | alanine racemase | - |
| MID01_RS02595 (509868) | 509868..510149 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| MID01_RS02600 (510154) | 510154..510504 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| MID01_RS02605 (510619) | 510619..511443 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| MID01_RS02610 (511448) | 511448..511813 | + | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| MID01_RS02615 (511817) | 511817..512218 | + | 402 | WP_003246640.1 | serine/threonine-protein kinase RsbT | - |
| MID01_RS02620 (512230) | 512230..513237 | + | 1008 | WP_003234295.1 | phosphoserine phosphatase RsbU | - |
| MID01_RS02625 (513299) | 513299..513628 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| MID01_RS02630 (513625) | 513625..514107 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| MID01_RS02635 (514073) | 514073..514861 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| MID01_RS02640 (514861) | 514861..515460 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T235969 WP_003156187.1 NZ_CP092369:510154-510504 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T235969 NZ_LR134520:88110-88457 [Streptococcus agalactiae]
TTGGTATCTGATAATAAAACCCATAGCCTTATTATCCCTGAAACCGTACAAGAACAATTACAGGAGATAAAAAGCTATAT
TGAAACTACTTACTTTTCGGAGCAGGCAGGGGCTAACACCGTCAACAATATTCTTTACGGGCTAGAACGTCTTGAATTTT
TCCCAGAAGCAGGATTTAACGCAGATGACAGAGTAGGTGAAACTATTTACCCGCCACATAATACTCGTTGTATTGTTTTA
GGGGACTACCTAGCTTTTTATCACATTTTAGAAGACAGAAAAGCCGTTTTTGTATCTGATATTATCCATAGCAAACAAGA
CTATATCAAACTGTTTAAGAAAAAATAA
TTGGTATCTGATAATAAAACCCATAGCCTTATTATCCCTGAAACCGTACAAGAACAATTACAGGAGATAAAAAGCTATAT
TGAAACTACTTACTTTTCGGAGCAGGCAGGGGCTAACACCGTCAACAATATTCTTTACGGGCTAGAACGTCTTGAATTTT
TCCCAGAAGCAGGATTTAACGCAGATGACAGAGTAGGTGAAACTATTTACCCGCCACATAATACTCGTTGTATTGTTTTA
GGGGACTACCTAGCTTTTTATCACATTTTAGAAGACAGAAAAGCCGTTTTTGTATCTGATATTATCCATAGCAAACAAGA
CTATATCAAACTGTTTAAGAAAAAATAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT235969 WP_003225183.1 NZ_CP092369:509868-510149 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT235969 NZ_LR134520:87833-88120 [Streptococcus agalactiae]
ATGCACGCTTTAGAAAAAAATACGCAGGTCAACTTTAAAACCAACAGTGATTTACTAGAGAAAGCTAAAGCTATTATCGC
AGCCCAAAACCTTGACATGACAGCTAGCTTTAATTTGTTTTTAGAAAACATTGTACAAAATAAAGCCTTACCTTTTGAGA
CTGACACCGATAAAGAACGAGCTGAACTACTTGCAGGTTTACGAGCTGAAATTGCTAAAAGTTTTGACGATTTAGAGCAT
GGGCGGGTATACAACGCTAGTGAAGTGAGGGCTAACCTTGGTATCTGA
ATGCACGCTTTAGAAAAAAATACGCAGGTCAACTTTAAAACCAACAGTGATTTACTAGAGAAAGCTAAAGCTATTATCGC
AGCCCAAAACCTTGACATGACAGCTAGCTTTAATTTGTTTTTAGAAAACATTGTACAAAATAAAGCCTTACCTTTTGAGA
CTGACACCGATAAAGAACGAGCTGAACTACTTGCAGGTTTACGAGCTGAAATTGCTAAAAGTTTTGACGATTTAGAGCAT
GGGCGGGTATACAACGCTAGTGAAGTGAGGGCTAACCTTGGTATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|