Detailed information of TA system TA235968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 682366..682986
• Replicon: chromosome
• Accession: NZ_CP092365
• Organism: Mycolicibacillus parakoreensis strain DSM 45575

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MIU77_RS03345
• Protein ID: WP_240171647.1
• Coordinates: 682549..682986 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: MIU77_RS03340
• Protein ID: WP_240171646.1
• Coordinates: 682366..682545 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIU77_RS03330 (MIU77_03325)	678249..679829	+	1581	WP_240171644.1	serine hydrolase	-
MIU77_RS03335 (MIU77_03330)	679826..682318	+	2493	WP_240171645.1	HAD-IC family P-type ATPase	-
MIU77_RS03340 (MIU77_03335)	682366..682545	+	180	WP_240171646.1	antitoxin	Antitoxin
MIU77_RS03345 (MIU77_03340)	682549..682986	+	438	WP_240171647.1	SRPBCC family protein	Toxin
MIU77_RS03350 (MIU77_03345)	683033..683443	+	411	WP_240171648.1	hypothetical protein	-
MIU77_RS03355 (MIU77_03350)	683440..686355	-	2916	WP_240171649.1	RND family transporter	-
MIU77_RS03360 (MIU77_03355)	686352..686798	-	447	WP_240171650.1	MmpS family transport accessory protein	-
MIU77_RS03365 (MIU77_03360)	686795..687418	-	624	WP_240171651.1	TetR family transcriptional regulator	-
MIU77_RS03370 (MIU77_03365)	687504..687644	-	141	WP_240171652.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (3-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15682.31 Da
• Isoelectric Point: 10.2881

>T235968 WP_240171647.1 NZ_CP092365:682549-682986 [Mycolicibacillus parakoreensis]
VAKLSGAIDVPLPPEQAWGHAADLARYRDWLTIHRLWRSPLPDTLEKGTVVESIVEVKGMPNRIKWTIVHFKPPQAMTLN
GDGKGGVKIKLMAKVKPQGDASVVSFDVHLGGPALFGPIGMVVAGALRGDINESLNRFVTVFAPQ

Nucleotide

• Length: 438 bp

>T235968 NZ_LR134519:c1389714-1389433 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TATTATAAGCATTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGTAATC
TTAAAGGGCTGAGAGAATGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6340.08 Da
• Isoelectric Point: 4.5310

>AT235968 WP_240171646.1 NZ_CP092365:682366-682545 [Mycolicibacillus parakoreensis]
MGFLDKAKGLLSKNADKVDQAIDKAGDLVDDKTEGKYADTVDKVQEAAKKAIDKTDPES

Nucleotide

• Length: 180 bp

>AT235968 NZ_LR134519:c1390021-1389707 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTGCAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA