Detailed information of TA system TA235965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 307564..308224
• Replicon: chromosome
• Accession: NZ_CP092364
• Organism: Mycolicibacterium goodii strain ATCC 700504

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MI170_RS01595
• Protein ID: WP_073679992.1
• Coordinates: 307781..308224 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: MI170_RS01590
• Protein ID: WP_073679993.1
• Coordinates: 307564..307770 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MI170_RS01580 (MI170_26195)	303533..305137	+	1605	WP_240173551.1	serine hydrolase	-
MI170_RS01585 (MI170_26190)	305151..307538	+	2388	WP_240173550.1	cation-translocating P-type ATPase	-
MI170_RS01590 (MI170_26185)	307564..307770	+	207	WP_073679993.1	antitoxin	Antitoxin
MI170_RS01595 (MI170_26180)	307781..308224	+	444	WP_073679992.1	SRPBCC family protein	Toxin
MI170_RS01600 (MI170_26175)	308303..309784	-	1482	WP_214386358.1	long-chain fatty acid--CoA ligase	-
MI170_RS01605 (MI170_26170)	309781..310554	-	774	WP_100519030.1	SDR family oxidoreductase	-
MI170_RS01610 (MI170_26165)	310555..312096	-	1542	WP_240173549.1	AMP-binding protein	-
MI170_RS01615 (MI170_26160)	312098..312835	-	738	WP_073679989.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16142.78 Da
• Isoelectric Point: 9.9432

>T235965 WP_073679992.1 NZ_CP092364:307781-308224 [Mycolicibacterium goodii]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRIRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGSIVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKALYAPSAT

Nucleotide

• Length: 444 bp

>T235965 NZ_LR134517:1228140-1228445 [Helicobacter pylori]
GTGTTGAAGCTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTGCTTTTGAATGGGTTTGATGATAGCGTTTT
AAAGGAAGTCATTCTAAGAAAGAAAGAACCGCTACCCCCACCATTTAAAGATCACGCTTTAAAGGGGGTTTGGAAGCCTT
TTAGAGAATGCCATATCAAGGCTGATGTTTTGCTTGTGTATTTAGTGAAAGATGATGAGCTTATTTTGTTAAGGTTAGGC
AGTCATAGCGAGTTGTTTTATAAACTACCCATAACGCTTAAAAAAAACGCAACAATCGCATGCTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7475.29 Da
• Isoelectric Point: 7.0091

>AT235965 WP_073679993.1 NZ_CP092364:307564-307770 [Mycolicibacterium goodii]
MGFLDKAKDLLSKNADKVDTVIDKAGDIVDQKTQGKYAQHVDKAQDAAKNAIRKDNPGNPDNPQEPQR

Nucleotide

• Length: 207 bp

>AT235965 NZ_LR134517:1227971-1228159 [Helicobacter pylori]
TTGAATAAAATCTTACAAGAGATCAACAAGAGGGCTAATGATTGTTGTTTTGGGCATGAAACCCCAAATATTGAAACGCA
ACAAGCCATGAGAGAAGCAGATAACAAAGAAACAGATTTGTTCGTTGAGGATTTTTCTAGTTATAGCAATGAAAGAAAAA
AGGCTTTAGGTGTTGAAGCTCAATCTTAA