Detailed information of TA system TA235963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5033192..5033848
• Replicon: chromosome
• Accession: NZ_CP092362
• Organism: Mycolicibacterium crocinum strain JCM 16369

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MI149_RS24530
• Protein ID: WP_240177510.1
• Coordinates: 5033192..5033638 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: MI149_RS24535
• Protein ID: WP_240177511.1
• Coordinates: 5033642..5033848 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MI149_RS24505 (MI149_24495)	5029899..5030783	+	885	WP_240177508.1	hypothetical protein	-
MI149_RS24510 (MI149_24500)	5030780..5031667	-	888	WP_071949333.1	amidohydrolase family protein	-
MI149_RS24515 (MI149_24505)	5031776..5032435	+	660	WP_240177509.1	response regulator transcription factor	-
MI149_RS24520 (MI149_24510)	5032437..5032661	-	225	WP_225933532.1	hypothetical protein	-
MI149_RS24525 (MI149_24515)	5032805..5033188	+	384	WP_096312224.1	hypothetical protein	-
MI149_RS24530 (MI149_24520)	5033192..5033638	-	447	WP_240177510.1	SRPBCC family protein	Toxin
MI149_RS24535 (MI149_24525)	5033642..5033848	-	207	WP_240177511.1	antitoxin	Antitoxin
MI149_RS24540 (MI149_24530)	5033852..5036251	-	2400	WP_240177512.1	HAD-IC family P-type ATPase	-
MI149_RS24545 (MI149_24535)	5036248..5037834	-	1587	WP_240177513.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (9-55)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15978.65 Da
• Isoelectric Point: 10.1635

>T235963 WP_240177510.1 NZ_CP092362:c5033638-5033192 [Mycolicibacterium crocinum]
MAKLSVSVDVPLPPEQAWQHASDLSRYKDWLTIHRVWRSKLPDTLEKGTVVESIVEVKGMPNRVKWEIVHFKAPEAMTLN
GAGVGGVKVKLIGKVRAHDKDAGSSVVTMDVHLGGAALFGPIGMVVAGALKGDIKESLNRFVTVFAPS

Nucleotide

• Length: 447 bp

>T235963 NZ_LR134517:21354-22094 [Helicobacter pylori]
ATGGAAACTAAAGAAGAAGATAAAGATAAAAAGCTAGAAGAAATGATTGTATTGCTGTGCGAAAAGGGGGATTTGTCTAG
TCAAACAGATCAAATCATCAAAGATCTCAAAGAAATCTATGAAGGAGAATACAGGCATAAATACTCAAAAATTACAACTA
TTATTTTAAATTCTACAAGGGATAAAGAGCAAGCCTTTATGACGCTCGCACAAAATATAAGGACACTCAAAGAGATTCAA
GATAATAAAGAAGTTGAAAGCATTAAACCAAAATTAGAAAAGCTTTATGATCACATGAATTTAGAGTGTATCAGACTGCA
AGATTTTGATGAGAAAATGAGTAGAGTTAAAGATGTTTCTAATAAGCTAGAAGATGATCTAAATAAAAACTATAAAAAAT
TAAGCGAAGAATTGAATAAGCAACAAACACAATACATAACCATTTTAGGTATTTTTGCTTCTATTGTTTTAACATTTGTT
GGGGGATTAGCGTTTTCCACTTCTGTTTTATCAAATATTGATAAAGCGAACGCCTATCGCTTGGTTTTTGTTATGGCTTT
TATAGCTTTATTTTTTGGGAATATTTTGTATCTGCTTTTTTCTTTTTTATCAAAAGTATCCTTATCAAAAGAGAAAAAAG
ATAAACAAGAAAATTTTTGCAAAAAACCCATGTTTTGGTTTAATCTAATAGTTACAATTCTATTTGTGATTGGTTTTTGT
GGAGAATTGCATATAATATAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7402.14 Da
• Isoelectric Point: 5.0152

>AT235963 WP_240177511.1 NZ_CP092362:c5033848-5033642 [Mycolicibacterium crocinum]
MGFLDKALGKTKEVLGSNADKVEQAIDKAGDIIDQKTQGKYSSTVDRVQDAAKNYVDSNRTDKGEQPN

Nucleotide

• Length: 207 bp

>AT235963 NZ_LR134517:20927-21364 [Helicobacter pylori]
ATGGTCAATACTTTGGAACTAGCACAATATATTCTTAAGCATTCAAACAAAGAATTGAGTAACTTAGAATTACAAAAAAC
TTTGTATTTCACAGAGCTTGATTATATCAAAAAGTTTGACAAACACCTAGTTTCTGATGATTTTGAAGCTTGGAAATACG
GGCCTGTTGCAAGAGAAGTGTATTATGAATACCGTAATTATGGTGCCAATTCCATTGACAAACCCAAAGAAGAAACTCTC
TCGCAAAATCTTAGAAAAGATGAACTTGAAACCATCAACCACTCCATAGAAAAATGCAACGAAAAATCCTATTGGGACTT
AGTGAAAGAGAGTCATAAAGAAAATGGCGCTTGGCATAAAAGCTTCAAAGAAGACAGAAAAGAAATCATTAGCAAAGATT
TGATCAAACAAGAAGCAAAGCAAACAAATGGAAACTAA