Detailed information of TA system TA235960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 1052491..1053068
• Replicon: chromosome
• Accession: NZ_CP092362
• Organism: Mycolicibacterium crocinum strain JCM 16369

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MI149_RS05025
• Protein ID: WP_240178903.1
• Coordinates: 1052685..1053068 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MI149_RS05020
• Protein ID: WP_090339709.1
• Coordinates: 1052491..1052688 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MI149_RS04995 (MI149_30240)	1048453..1048767	+	315	WP_262871739.1	hypothetical protein	-
MI149_RS05000 (MI149_04980)	1049191..1050225	-	1035	WP_240178899.1	hypothetical protein	-
MI149_RS05005 (MI149_04985)	1050319..1050963	+	645	WP_240178900.1	NUDIX domain-containing protein	-
MI149_RS05010 (MI149_04990)	1050984..1052096	-	1113	WP_240178901.1	plasmid pRiA4b ORF-3 family protein	-
MI149_RS05015 (MI149_04995)	1052146..1052394	-	249	WP_240178902.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
MI149_RS05020 (MI149_05000)	1052491..1052688	+	198	WP_090339709.1	antitoxin Phd	Antitoxin
MI149_RS05025 (MI149_05005)	1052685..1053068	+	384	WP_240178903.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MI149_RS05030 (MI149_05010)	1053263..1054513	+	1251	WP_240178904.1	cytochrome P450	-
MI149_RS05035 (MI149_05015)	1054578..1055003	+	426	WP_240178905.1	cupin domain-containing protein	-
MI149_RS05040 (MI149_05020)	1055060..1055275	-	216	WP_240178906.1	DUF4926 domain-containing protein	-
MI149_RS05045 (MI149_05025)	1055289..1056104	-	816	WP_262871740.1	hypothetical protein	-
MI149_RS05050 (MI149_05030)	1056635..1056922	-	288	WP_240178908.1	WXG100 family type VII secretion target	-
MI149_RS05055 (MI149_05035)	1056919..1057236	-	318	WP_240178909.1	WXG100 family type VII secretion target	-
MI149_RS05060 (MI149_05040)	1057402..1057977	+	576	WP_240180295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1048949..1059600	10651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (21-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13512.04 Da
• Isoelectric Point: 3.8512

>T235960 WP_240178903.1 NZ_CP092362:1052685-1053068 [Mycolicibacterium crocinum]
VTEYLDRDDVLVAGAAAVGVELRVADYGLLDAAVARPQATAFGVDAYPDLFTKAAALLHSLARNHALVDGNKRTAWAAAW
TFLYINGVELDPDFDVDDAEDFMNAVAVQGDLQLAEIGQTLQAFADG

Nucleotide

• Length: 384 bp

>T235960 NZ_LR134516:c260273-260007 [Neisseria animaloris]
ATGAAAGCGATTGAGTTCAGTAACGCATTCAAACGCGATTTCAAAAAATATCCTGCGCTGGCTCTGTCAACGCGCTGGAT
TGAAGCACTCTACTGTATATGCAGCGAACAAACCCTGCCGCCTAAATTTTGCGACCACGCACTCACGGGCAACTGGCACG
GCTTTCGCGACTGCCACATCGAGCCTGATTTGGTGCTGATTTACCGCCATACGGAAAACACAGTCGAACTGGCACGAATC
GGCACGCACTCCGCGCTGTTCGGCTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7158.11 Da
• Isoelectric Point: 11.0635

>AT235960 WP_090339709.1 NZ_CP092362:1052491-1052688 [Mycolicibacterium crocinum]
MPALNVNFSDAEMEQLRTAAAAGELSLRAFVHRAALDAASDHKRRVREGARIVAERSAELNRRLA

Nucleotide

• Length: 198 bp

>AT235960 NZ_LR134516:c260526-260260 [Neisseria animaloris]
ATGGCTGCCAATGCACTTGTGAGCGTACGCATCAACGAAGATGTGAAAAACCAAGCCAGCGCAGTATTGGCCGGTGCGGG
TTTGACCATCTCCGACGTCATCCGCATGACGCTGACCAAAATCGCCACCGAGCAACGCTTCAGCTTCGACTACCAACCCA
ACGTCGAAACCGCACGCGTTTTAACTGCCATGCAAAACGGCACGGAAACCTTGCATCGCGCCGACAGTGTGGAAGCATTG
ATGGAACGGCTGAATGAAAGCGATTGA