Detailed information of TA system TA235949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1008809..1009390
• Replicon: chromosome
• Accession: NZ_CP092358
• Organism: Wielerella bovis strain 09LB414

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MIS46_RS05120
• Protein ID: WP_249443592.1
• Coordinates: 1009061..1009390 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MIS46_RS05115
• Protein ID: WP_249443591.1
• Coordinates: 1008809..1009054 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIS46_RS05095 (MIS46_05095)	1004565..1004741	-	177	WP_249443588.1	hypothetical protein	-
MIS46_RS05100 (MIS46_05100)	1004806..1007472	+	2667	WP_249443589.1	aminopeptidase N	-
MIS46_RS05105 (MIS46_05105)	1007545..1008099	-	555	WP_249443590.1	hypothetical protein	-
MIS46_RS05110 (MIS46_05110)	1008222..1008608	+	387	Protein_982	helix-turn-helix transcriptional regulator	-
MIS46_RS05115 (MIS46_05115)	1008809..1009054	+	246	WP_249443591.1	antitoxin	Antitoxin
MIS46_RS05120 (MIS46_05120)	1009061..1009390	+	330	WP_249443592.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MIS46_RS05125 (MIS46_05125)	1009835..1011283	+	1449	WP_249443793.1	metalloprotease TldD	-
MIS46_RS05130 (MIS46_05130)	1011641..1012654	-	1014	WP_249443593.1	CAP domain-containing protein	-
MIS46_RS05135 (MIS46_05135)	1012793..1014226	-	1434	WP_249443594.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-107)

Antitoxin

• Antitoxin PemI-like (4-81)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12182.05 Da
• Isoelectric Point: 8.8278

>T235949 WP_249443592.1 NZ_CP092358:1009061-1009390 [Wielerella bovis]
MERGDIFLVNLDPTTGHEQQGTRPVLIVSPKAFNDLTQVPIVLPITTKSQFARTQGFAVHLDERTKTQGIIRTDQPRALD
INARNGRKLETLPIDLMNEVLAKMATFLS

Nucleotide

• Length: 330 bp

>T235949 NZ_LR134503:c2251187-2250888 [Kaistella jeonii]
ATGTACTTTATTGAAAAAACAGAAGAGTTTGACAAATGGTTTAGAAAATTGAATGATTTAAGAGCAAAAGCAAAAATTTT
GTTTCGAATTCAGAAATTAGAAACTGACGAACATTTCGGCGATTTTAAACCAGTTGGAAATGGAATTAATGAGTTGAAGA
TTAATTACGCAAAAGGTTATAGAATTTACTTTAAGGAGACTGAGGGAAAAATCATTATTCTTCTAATTGGCGGAGATAAA
TCAACTCAACAAAAGGACATTGAAAAAGCGTTTCAAATCTGGAATAAATTAAAGAAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8907.19 Da
• Isoelectric Point: 4.9189

>AT235949 WP_249443591.1 NZ_CP092358:1008809-1009054 [Wielerella bovis]
MYTVSLRKVGGSTMLAIPPALLQSLDLGKQVHLHTENGRLIIEPARPQYTLAELLAQCDPNAPRDAESQTWLDAPAIGQE
W

Nucleotide

• Length: 246 bp

>AT235949 NZ_LR134503:c2250882-2250598 [Kaistella jeonii]
ATGAATACAACAAAATTTGACATTGCAGATTACTTGGACAATAACGAAATGATTGCAGAATATCTTAATACTGTTCTGGA
AGAAGGAAATGACTCAGATATAATTGTCGCAATTGGAACTGTTGCTAAAGCGATTGGAATGACTAAAATTGCAGAAGAAA
CAGGACTTAGTAGACCGAGTTTATACAAAGCGTTATCAGACGGTGCTAAACCACAATTCGCAACAATAATGAAAGTTCTT
AAAGCCGTTGGTGGACAATTACATGTGAATCCTACTACAGCGTAA