Detailed information of TA system TA235946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 422783..423381
• Replicon: plasmid pRSpC104a
• Accession: NZ_CP092352
• Organism: Rhizobium sp. C104

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MF410_RS30065
• Protein ID: WP_097536147.1
• Coordinates: 422783..423076 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2A6K8H2
• Locus tag: MF410_RS30070
• Protein ID: WP_062578764.1
• Coordinates: 423073..423381 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF410_RS30040 (MF410_30040)	418100..418333	+	234	WP_239620736.1	hypothetical protein	-
MF410_RS30045 (MF410_30045)	418642..419874	+	1233	WP_131710562.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MF410_RS30050 (MF410_30050)	419856..421851	+	1996	Protein_374	glycosyltransferase	-
MF410_RS30055 (MF410_30055)	421949..422368	-	420	WP_097629453.1	type II toxin-antitoxin system VapC family toxin	-
MF410_RS30060 (MF410_30060)	422365..422619	-	255	WP_003550301.1	plasmid stabilization protein	-
MF410_RS30065 (MF410_30065)	422783..423076	-	294	WP_097536147.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MF410_RS30070 (MF410_30070)	423073..423381	-	309	WP_062578764.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MF410_RS30075 (MF410_30075)	423578..425104	+	1527	WP_130713392.1	IS21 family transposase	-
MF410_RS30080 (MF410_30080)	425094..425906	+	813	WP_239620351.1	IS21-like element helper ATPase IstB	-
MF410_RS30085 (MF410_30090)	426170..426683	-	514	Protein_381	fasciclin domain-containing protein	-
MF410_RS30090 (MF410_30095)	426877..427530	-	654	WP_097624209.1	ChrR family anti-sigma-E factor	-
MF410_RS30095 (MF410_30100)	427527..427973	-	447	WP_239620738.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..828029	828029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10568.08 Da
• Isoelectric Point: 5.7996

>T235946 WP_097536147.1 NZ_CP092352:c423076-422783 [Rhizobium sp. C104]
VKLTWSAFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLVDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDSLPTS

Nucleotide

• Length: 294 bp

>T235946 NZ_LR134503:c1868607-1868350 [Kaistella jeonii]
ATGAAATATGTTTTTGTAGATGAATCCTGGGAAGATTACTTGTACTGGCAGAAAAACGATAAAAAGAAACTCAAACGAAT
CAACGATTTGCTAAAAGATATTTCAAGAAATCCTTTCGATGGTATTGGAAAGCCAGAACCTTTAAAGCATAAATATTCTG
GATTTTGGTCTAGAAGAATTGATGATGAACATCGATTAATTTATAAGTATGAAGAAGATCAAATTTTAATTGCGAAGTGC
AGATTTCATTATGACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10998.32 Da
• Isoelectric Point: 5.1570

>AT235946 WP_062578764.1 NZ_CP092352:c423381-423073 [Rhizobium sp. C104]
MAANALVQTRIDAEVRDRASAVLGNMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDVSDD
EVEAHFAERRAAARLNAGARKS

Nucleotide

• Length: 309 bp

>AT235946 NZ_LR134503:c1868863-1868609 [Kaistella jeonii]
ATGATAATTGCAAGCGTTTCAGACTTTAGAAAAGATATAAAATCTTATTTGGACAGGGTTGCAAAGAATTTTGAAACTCT
AATTATTAATCGTGGAAAAGAGGATGGTATTGTAGTGATGTCACTTGAAGAATATAATTCATTGATGATTACTAATCACG
AATTATCTTCCCGAAAAAATGAGCTGAGATTAGATGCAGCCATTGGAAAACTAAAAAGTGGGAAATCATTTTCTAAAAAT
TTAATAGAAGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A6K8H2