Detailed information of TA system TA235943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 1674699..1675393
• Replicon: plasmid pRGM101b
• Accession: NZ_CP092346
• Organism: Rhizobium gallicum strain M101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L2W42_RS31820
• Protein ID: WP_239600612.1
• Coordinates: 1674953..1675393 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L2W42_RS31815
• Protein ID: WP_239600611.1
• Coordinates: 1674699..1674950 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2W42_RS31790 (L2W42_31790)	1670083..1671909	+	1827	WP_239600604.1	phosphomethylpyrimidine synthase ThiC	-
L2W42_RS31795 (L2W42_31795)	1671911..1672894	+	984	WP_239600606.1	glycine oxidase ThiO	-
L2W42_RS31800 (L2W42_31800)	1672891..1673088	+	198	WP_239600607.1	sulfur carrier protein ThiS	-
L2W42_RS31805 (L2W42_31805)	1673092..1673865	+	774	WP_239600609.1	thiazole synthase	-
L2W42_RS31810 (L2W42_31810)	1673862..1674473	+	612	WP_239600610.1	thiamine phosphate synthase	-
L2W42_RS31815 (L2W42_31815)	1674699..1674950	+	252	WP_239600611.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L2W42_RS31820 (L2W42_31820)	1674953..1675393	+	441	WP_239600612.1	PIN domain-containing protein	Toxin
L2W42_RS31825 (L2W42_31825)	1675494..1676192	-	699	WP_239600613.1	transposase	-
L2W42_RS31830 (L2W42_31830)	1676189..1676518	-	330	WP_239600614.1	helix-turn-helix domain-containing protein	-
L2W42_RS31835 (L2W42_31835)	1676585..1676914	+	330	Protein_1664	IS200/IS605 family transposase	-
L2W42_RS31840 (L2W42_31840)	1677142..1678538	-	1397	Protein_1665	dihydrolipoyl dehydrogenase	-
L2W42_RS31845 (L2W42_31845)	1678544..1679803	-	1260	WP_239600615.1	dihydrolipoamide acetyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..2542150	2542150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-129)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-65)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 15901.23 Da
• Isoelectric Point: 6.3317

>T235943 WP_239600612.1 NZ_CP092346:1674953-1675393 [Rhizobium gallicum]
MYLLDTNIVSADAPTKRQVGVEAFTAWMRAHGDRLYLSTITIAEIEAGIARAVRIAATTKAENLGRWLSAVEHFYAGRIL
PFGIEEARHAGLILDRARVHDPGFEDIAIAATAAAHGLTVLTTNERDFEPLGVPLANPLKQLPTMQ

Nucleotide

• Length: 441 bp

>T235943 NZ_LR134495:c2090593-2090204 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATACAAATGTAGTGGCAAAATTAAAATCCATTAA
TCCTGAGAAGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGCAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGAGTGCAGGATCAAATGATTGCTAGCCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATTTAAAATTAGAAAATTGGTTGCACTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9127.48 Da
• Isoelectric Point: 10.0863

>AT235943 WP_239600611.1 NZ_CP092346:1674699-1674950 [Rhizobium gallicum]
MKEVQLKDAKATLSAVVDRAVAGEPTVITRHGRKEAVLVSFEEWQRISKVPSFADLLLAFPGEPADIPERSRKGARVLSE
NGR

Nucleotide

• Length: 252 bp

>AT235943 NZ_LR134495:c2090854-2090594 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAATGGGAGAAACCAAGCGGTACGTTTACCGGTTGAATTTGAGTTCGATGCCGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATGCCGAGCATTGGCAAAAAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGACACAAAAGAGAGAAACCAATCCTTTGAGCAAAGG
GATCCTTTTGAGGTGGCTTAA