Detailed information of TA system TA235942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 1603440..1604310
• Replicon: plasmid pRGM101b
• Accession: NZ_CP092346
• Organism: Rhizobium gallicum strain M101

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L2W42_RS31510
• Protein ID: WP_239600560.1
• Coordinates: 1603804..1604310 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1L5NV54
• Locus tag: L2W42_RS31505
• Protein ID: WP_074072035.1
• Coordinates: 1603440..1603811 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2W42_RS31490 (L2W42_31490)	1599741..1601720	+	1980	WP_239600554.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
L2W42_RS31495 (L2W42_31495)	1601720..1602589	+	870	WP_239600557.1	hydroxymethylglutaryl-CoA lyase	-
L2W42_RS31500 (L2W42_31500)	1602586..1603377	+	792	WP_239600559.1	crotonase/enoyl-CoA hydratase family protein	-
L2W42_RS31505 (L2W42_31505)	1603440..1603811	+	372	WP_074072035.1	DUF2384 domain-containing protein	Antitoxin
L2W42_RS31510 (L2W42_31510)	1603804..1604310	+	507	WP_239600560.1	RES domain-containing protein	Toxin
L2W42_RS31515 (L2W42_31515)	1604639..1606135	-	1497	WP_239603572.1	DegQ family serine endoprotease	-
L2W42_RS31520 (L2W42_31520)	1606445..1607857	-	1413	WP_239600561.1	FAD/NAD(P)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..2542150	2542150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (7-161)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18941.81 Da
• Isoelectric Point: 7.4154

>T235942 WP_239600560.1 NZ_CP092346:1603804-1604310 [Rhizobium gallicum]
MPETVVHLWRAFVPRWAHAPLSGEGASRFGGRWNRVGAPTIYAACELSTAWAEYNQGFVQHPAMIVQLELKGACLADTTD
QMLLRKFDVTADIHRCEWRMLMDESKVPATHHLRERLIASRYHGAIYPSFMSLGGTCVALWEWNMPGAPELRAIDPEGRL
PKSPASWL

Nucleotide

• Length: 507 bp

>T235942 NZ_LR134495:1848138-1848557 [Mannheimia haemolytica]
ATGTATTTACTTGATACCAATATCATTAGCGAAATCCGAAAAATCAATACAGGCAAAGCCAATCAGGGGGTTATTGATTG
GCTTTCCGCCGTTCCAGGAAATCAACTCTATACAAATGCAATCGTATTAATGGAATTAGAACGAGGTGTATCGGCAAAAG
AGCGTAAAGATAAACAGCAAGGGGCGATATTACGCAAATGGTTAGAGCAAGTGATTAAACCGTTTTTTGCACAGAGAACG
CTCACGATTGATGCACAAACCTCTCAAGTGTGTGCCAAACTACACATTCCGGATCACGCCCCTGAAAATGATGCATGGAT
TGCGGCAAATGCAATTCAGCATAATTTTATTTTAGTCACACACAATACGAATAATTTTGCCCGTACAGGCGTAAAGCTAC
TCGATCCATTTAAAAGCTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13057.91 Da
• Isoelectric Point: 10.1453

>AT235942 WP_074072035.1 NZ_CP092346:1603440-1603811 [Rhizobium gallicum]
MAATGLSNVAARFGDEHSSFLSARRVAEQLGVTLSELARLIGVARNTLAAKSGARKVDNALSNVVRILAMASEMAGDENR
AVIWFKHQPIPGWGGKTAFDLVGEGKSDRVLAYLEAVRSGVYA

Nucleotide

• Length: 372 bp

>AT235942 NZ_LR134495:1847878-1848138 [Mannheimia haemolytica]
ATGACTATTATGACCAGCAGAGAGTTTAATCAGCACTTAAGCCAAGCACAAAAGGCAGCACAATTTTCACCTGTAATTAT
CACTAATCGAGGTGAACCGGCTTTTGTATTAATGAGTTATACCGAATATCAAAAGACGCATATAAAGCCTGTCAGAAGTG
CTTTAGAAGCACTAACACCAATAGACAACGAAGATATTGAGTTAGTCTTACAACCTCGCAGTACGAATCGAAGAAAGCTC
TCTATTTTTGAGGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L5NV54