Detailed information of TA system TA235941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1285318..1285835
• Replicon: plasmid pRGM101b
• Accession: NZ_CP092346
• Organism: Rhizobium gallicum strain M101

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L2W42_RS29965
• Protein ID: WP_239600320.1
• Coordinates: 1285318..1285536 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L2W42_RS29970
• Protein ID: WP_239600321.1
• Coordinates: 1285536..1285835 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2W42_RS29945 (L2W42_29945)	1280715..1281707	+	993	WP_239600317.1	tripartite tricarboxylate transporter substrate-binding protein	-
L2W42_RS29950 (L2W42_29950)	1281765..1282259	+	495	WP_239600318.1	tripartite tricarboxylate transporter TctB family protein	-
L2W42_RS29955 (L2W42_29955)	1282277..1283791	+	1515	WP_074072324.1	tripartite tricarboxylate transporter permease	-
L2W42_RS29960 (L2W42_29960)	1283830..1285239	+	1410	WP_239600319.1	flagellar biosynthesis protein FlgA	-
L2W42_RS29965 (L2W42_29965)	1285318..1285536	-	219	WP_239600320.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2W42_RS29970 (L2W42_29970)	1285536..1285835	-	300	WP_239600321.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
L2W42_RS29975 (L2W42_29975)	1285989..1286888	-	900	WP_239600322.1	LysR family transcriptional regulator ArgP	-
L2W42_RS29980 (L2W42_29980)	1286985..1287593	+	609	WP_239600323.1	LysE/ArgO family amino acid transporter	-
L2W42_RS29985 (L2W42_29985)	1287769..1288407	+	639	WP_239600325.1	LysE family translocator	-
L2W42_RS29990 (L2W42_29990)	1288425..1289315	+	891	WP_239600326.1	dihydrodipicolinate synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..2542150	2542150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-63)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-55)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8294.39 Da
• Isoelectric Point: 5.6566

>T235941 WP_239600320.1 NZ_CP092346:c1285536-1285318 [Rhizobium gallicum]
MRLVWSQYALDDRDSIFSYIEAENPRAAVHVDEEVVRAARRLLEFPESGRPGRIAGTRELVYPAHTLYCAMQ

Nucleotide

• Length: 219 bp

>T235941 NZ_LR134495:495418-495840 [Mannheimia haemolytica]
ATGTATTTGCTGGATACAAACATCGTTTCTGAACTGCGGAAAGCGAGCAAAGGCAGAGCCGATCCAAACGTAATGCGTTG
GTTTTCAAAAGTAGAATTACAAGATTGTTATCTTTGTAGCACCGTTATTAGTGAAATACGTGTTGGCATTTTGGCAAAGA
AAAAGCGAGATGTGGAACAATACTATATTTTAAATGATTGGTTTGAAAATCAACTACTTACGCAGTTCTCAAATCGAATT
TTACCACTTGATTTAACGGCAGCATTACTCTGTGCAGAATTTCATATTCCTGATCGAAGCCCGATTAATGATGTCTATAT
TGCGGCTATCGCAAAAGCAAACCATTTAACCTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGCGTTTAT
TTAACCCATTTGAAATCGCATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10690.02 Da
• Isoelectric Point: 4.8311

>AT235941 WP_239600321.1 NZ_CP092346:c1285835-1285536 [Rhizobium gallicum]
MASNALVQTRIDAAVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELVSNSDVHDAWFRAKVLQALEDTRADIEDA
DADKHFSARRSAALLKGSD

Nucleotide

• Length: 300 bp

>AT235941 NZ_LR134495:495143-495418 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTATT
TATCACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAGCAAAGCCACAAACGCTGG
CAGATGTTTTTGCCTCCCCTATTGTTGAAGAAGATGATGTGGATATGGAAGATTTTGAGCAAATTTTGCGAGATATTCGC
CAAAATTCAAAATGTCGCCCCGTGGAGTTTGATTAA