Detailed information of TA system TA235937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3275362..3276379
• Replicon: chromosome
• Accession: NZ_CP092344
• Organism: Rhizobium gallicum strain M101

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: L2W42_RS16605
• Protein ID: WP_275593287.1
• Coordinates: 3275362..3275952 (-)
• Length: 197 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: L2W42_RS16610
• Protein ID: WP_183906422.1
• Coordinates: 3275933..3276379 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2W42_RS16580 (L2W42_16580)	3270709..3271248	-	540	WP_239596203.1	SH3 domain-containing protein	-
L2W42_RS16585 (L2W42_16585)	3271435..3272436	+	1002	WP_239596205.1	D-glycerate dehydrogenase	-
L2W42_RS16590 (L2W42_16590)	3272433..3272906	-	474	WP_239596208.1	GNAT family N-acetyltransferase	-
L2W42_RS16595 (L2W42_16595)	3272903..3273727	-	825	WP_239596210.1	molybdopterin-synthase adenylyltransferase MoeB	-
L2W42_RS16600 (L2W42_16600)	3273724..3274896	-	1173	WP_239599656.1	DNA replication/repair protein RecF	-
L2W42_RS16605 (L2W42_16605)	3275362..3275952	-	591	WP_275593287.1	PIN domain-containing protein	Toxin
L2W42_RS16610 (L2W42_16610)	3275933..3276379	-	447	WP_183906422.1	helix-turn-helix domain-containing protein	Antitoxin
L2W42_RS16620 (L2W42_16620)	3276846..3277229	-	384	WP_022713204.1	TIGR02300 family protein	-
L2W42_RS16625 (L2W42_16625)	3277513..3277734	+	222	Protein_3290	FMN-binding negative transcriptional regulator	-
L2W42_RS16630 (L2W42_16630)	3277731..3278375	+	645	WP_239596215.1	(d)CMP kinase	-
L2W42_RS16635 (L2W42_16635)	3278552..3280252	+	1701	WP_022713201.1	30S ribosomal protein S1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (13-119)

Antitoxin

• SinI-like, DNA-binding domain (78-122)

Sequences

Toxin

Protein

• Length: 197 a.a.
• Molecular weight: 22032.07 Da
• Isoelectric Point: 5.0545

>T235937 WP_275593287.1 NZ_CP092344:c3275952-3275362 [Rhizobium gallicum]
IWGWAIKKSSYTVILDACVLYPAPLRDFLMELAAAGQFRAKWSDEIHDEWTRNVLKNRDDLTIEQLGRTKDLMNAAVPDS
VVDGYSDLVPALALPDPDDRHFLAAAIVSASDAIVTFNLSDFPRKTVSQYDIEVLHPDDFIHHQFGLNNAGVIIAAQHCR
ARLKNPPKTASEYLETLERQGLPKTVAELREYLFAI

Nucleotide

• Length: 591 bp

>T235937 NZ_LR134494:c3958297-3957911 [Serratia quinivorans]
ATGTATATGTTTGATACCAATACGGTCAGCCAGCTCTTTCGCAGGCACCCTCGTCTGCTCGGCTTAATGGAAAAGATCCC
GCCTTCTGCGGTCTGCATTTCCAGTATTACCGAGGCTGAATTGCTCTATGGCGTCGCCAAACGGCAGAGTCTGACGCTGA
AAGCGACGGTTGCGGCATTCCTTGATTCCGTGACGGTCTATGCCTGGGACAGTGAAGCCGCGCATTGCTATGGCGCTATG
CGGGCGGAGATGACAAAAAACGGGCGGGTGATGAGGGCTTTGGATCAGCTTATTGCCGCACACGCCCAAAGCCGCGGTGC
GACGATTGTGACTAACGATAAGGCGTTCGCCATGGTACCAGGCCTGGCAGTGGAAGACTGGACTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16618.01 Da
• Isoelectric Point: 5.9324

>AT235937 WP_183906422.1 NZ_CP092344:c3276379-3275933 [Rhizobium gallicum]
MSQLLERPILPSADDTELAAEASRYISRAKHEGAELRVQVDGGELLKLPKAVSELLYHLLTEMAQGNAVTLIPVHAELTT
QEAADHLNVSRPYLIKLLEEGKIPFHMVGSHRRIKFSDLDGFRRKAEDERKRLMDELASQAQDLGMGY

Nucleotide

• Length: 447 bp

>AT235937 NZ_LR134494:c3958563-3958300 [Serratia quinivorans]
ATGGAAAGAGTCGCGAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGATTACCCGTGGAATTCGAATTCGATACAGA
TCGCGTGTATATCCGCCGCGATCGGGAGGGAAACGTGATTTTATCGAAATATCCGGCGAAACCTGATAGTTGGGCTCCGC
TGCTGCAAATGATCGCACAAACGCAGGTACCGCAGGACTTCCTGACCGCGGAGGATCGTCAGCAGGGGATGGCCACCCGC
GATCCCTTTGCCGGGGAAGAATAA