Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 86158..86683 Replicon plasmid pKPN20-1
Accession NZ_CP092337
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag MG291_RS26195 Protein ID WP_013023785.1
Coordinates 86378..86683 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag MG291_RS26190 Protein ID WP_006788213.1
Coordinates 86158..86376 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS26180 (MG291_26180) 83839..84867 - 1029 WP_001568023.1 Abi family protein -
MG291_RS26185 (MG291_26185) 85008..85577 - 570 WP_016162066.1 hypothetical protein -
MG291_RS26190 (MG291_26190) 86158..86376 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MG291_RS26195 (MG291_26195) 86378..86683 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
MG291_RS26200 (MG291_26200) 86852..87247 + 396 WP_017899885.1 hypothetical protein -
MG291_RS26205 (MG291_26205) 87274..87588 + 315 WP_053389906.1 hypothetical protein -
MG291_RS26210 (MG291_26210) 87599..88615 + 1017 WP_017899884.1 hypothetical protein -
MG291_RS26215 (MG291_26215) 88813..89607 + 795 WP_004197635.1 site-specific integrase -
MG291_RS26220 (MG291_26220) 90047..90349 - 303 WP_071571079.1 hypothetical protein -
MG291_RS26225 (MG291_26225) 90346..90972 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / ARR-3 / rmtF - 1..212639 212639


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T235934 WP_013023785.1 NZ_CP092337:86378-86683 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T235934 NZ_LR134494:c1744907-1744623 [Serratia quinivorans]
ATGACTTATAACCTTGAATTCGAAGAACACGCACTGAAGGAATTTAAGAAGTTGGCTCCGGTGATCCGGGAACAGTTCAA
GAAAAAATTGGTGGCCGTGTTAGAGAACCCACTGGTTCCCGCCAATAAGCTATCCGGCCTGCCTGATTGCTATAAAATAA
AACTAAGGGCTTCTGGTTACCGTCTGGTCTATCGCGTCATCGGCGAAGAGATTGTGGTGCTGGTACTCTCGATAGGTAAA
CGTGAACGCTCAGAGGCCTATACTTCGGCCAAAAAACGCCTTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT235934 WP_006788213.1 NZ_CP092337:86158-86376 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT235934 NZ_LR134494:c1745148-1744897 [Serratia quinivorans]
ATGATCGTCCAGCCCATTCTGGCAGATTCAGCAGTCAGCATCAGCGATTTTAAAAAATCACCTAACGCAGCACTCAAAGA
AGCCCACGGACAGCCCGTTGCCGTGCTTACCAATGGGAAGATTTCCGGTTATTACGTCTCTCCTGAGACCTGGCAAGCCA
TTGCTGAATGCCTCGAGGATATTGAACTCGCTGAAATAGTCCGCTCACGGATGAGTGGCAAACGTATTAAGGTCAATCTG
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References