Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 22228..22871 Replicon plasmid pKPN20-1
Accession NZ_CP092337
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag MG291_RS25810 Protein ID WP_032425563.1
Coordinates 22455..22871 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag MG291_RS25805 Protein ID WP_016338373.1
Coordinates 22228..22458 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS25785 (MG291_25785) 17382..18161 - 780 WP_004213560.1 site-specific integrase -
MG291_RS25790 (MG291_25790) 18158..18979 - 822 WP_004213562.1 hypothetical protein -
MG291_RS25795 (MG291_25795) 19850..20803 + 954 WP_109549184.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
MG291_RS25800 (MG291_25800) 21096..22010 + 915 WP_126834912.1 Abi family protein -
MG291_RS25805 (MG291_25805) 22228..22458 + 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MG291_RS25810 (MG291_25810) 22455..22871 + 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
MG291_RS25815 (MG291_25815) 22912..23235 - 324 WP_085842301.1 hypothetical protein -
MG291_RS25820 (MG291_25820) 23253..23789 - 537 WP_223825639.1 hypothetical protein -
MG291_RS25825 (MG291_25825) 24448..25725 + 1278 WP_016338369.1 HlyD family secretion protein -
MG291_RS25830 (MG291_25830) 25715..27823 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / ARR-3 / rmtF - 1..212639 212639


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T235932 WP_032425563.1 NZ_CP092337:22455-22871 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T235932 NZ_LR134494:c949512-949222 [Serratia quinivorans]
GTGCCACAACTGATATGGACGCCTGCAGCGCTACAAGATATTCAGCGATTGTACCGATTTCTGGCGGAGAAAGACCTCAA
CTCCGCAAAGAGGGCCGTTGCCACCATCCGTCACAGCGTCAATATCCTCGCTCACCAACCGCAGGTAGGTCGCCCGGTTG
AAGAGATGGATACGGAATTTAGAGAATGGCCGATCGATTTCGGCAACAGTGGTTATATCGCGCTATATCATTTTGATGGC
CACACCGCAGTACTGCTGGCGGTGCGCCACCAAAGTGAAGCCGGCTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT235932 WP_016338373.1 NZ_CP092337:22228-22458 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT235932 NZ_LR134494:c949778-949500 [Serratia quinivorans]
ATGACAAGTGCAACATCGATTAAAATTGATGAACAACTGAAGCAGCGGGTGCAGCAAATTGCGGCTACACGCCAGCGCAG
CGCGCACTGGATAATGCGCGAGGCGATCCGCGAATATGTCGAACGAGAAGAAAAACGCGAAGCGCTGCGCAAAGATGCGT
TACAGGCATGGGAAGTCTATCAGGCAAATGGTAAACATCTGTCTCAGGAAGAGGCCGATAATTGGCTCATCAGCCTGGAA
AAAGGTGATGATACGGAGATCCCGGAGTGCCACAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References