Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5273657..5274282 Replicon chromosome
Accession NZ_CP092336
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MG291_RS25320 Protein ID WP_002882817.1
Coordinates 5273657..5274040 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MG291_RS25325 Protein ID WP_004150355.1
Coordinates 5274040..5274282 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS25305 (5271023) 5271023..5271925 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MG291_RS25310 (5271922) 5271922..5272557 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MG291_RS25315 (5272554) 5272554..5273483 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MG291_RS25320 (5273657) 5273657..5274040 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MG291_RS25325 (5274040) 5274040..5274282 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MG291_RS25330 (5274487) 5274487..5275404 + 918 WP_004181612.1 alpha/beta hydrolase -
MG291_RS25335 (5275418) 5275418..5276359 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MG291_RS25340 (5276404) 5276404..5276841 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MG291_RS25345 (5276838) 5276838..5277698 - 861 WP_004146232.1 virulence factor BrkB family protein -
MG291_RS25350 (5277692) 5277692..5278291 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T235931 WP_002882817.1 NZ_CP092336:c5274040-5273657 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T235931 NZ_LR134493:3794487-3794583 [Serratia rubidaea]
AACAAGCCCTGCAATAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT235931 WP_004150355.1 NZ_CP092336:c5274282-5274040 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT235931 NZ_LR134493:c3794583-3794470 [Serratia rubidaea]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTATTGCAGGGCTTGTTCAGCCATGCACTTTAAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References