Detailed information of TA system TA023593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 696085..696731
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: JTY_RS03115
• Protein ID: WP_003403122.1
• Coordinates: 696085..696477 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: JTY_RS03120
• Protein ID: WP_003403125.1
• Coordinates: 696474..696731 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS03100	691745..693273	+	1529	Protein_612	virulence factor Mce family protein	-
JTY_RS03105	693336..694478	+	1143	WP_003911253.1	virulence factor Mce family protein	-
JTY_RS03110	694483..696033	+	1551	WP_003403119.1	MCE family protein	-
JTY_RS03115	696085..696477	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
JTY_RS03120	696474..696731	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
JTY_RS03125	696914..698149	-	1236	WP_003403128.1	ATP-binding protein	-
JTY_RS03130	698400..698813	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
JTY_RS03135	698810..699046	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JTY_RS03140	699150..699656	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JTY_RS03145	699770..700300	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JTY_RS03150	700284..700979	-	696	WP_197044064.1	response regulator transcription factor	-
JTY_RS03155	701102..701413	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T23593 WP_003403122.1 NC_012207:c696477-696085 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T23593 NC_012207:c696477-696085 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT23593 WP_003403125.1 NC_012207:c696731-696474 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT23593 NC_012207:c696731-696474 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86