Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 696085..696731 Replicon chromosome
Accession NC_012207
Organism Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag JTY_RS03115 Protein ID WP_003403122.1
Coordinates 696085..696477 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag JTY_RS03120 Protein ID WP_003403125.1
Coordinates 696474..696731 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTY_RS03100 691745..693273 + 1529 Protein_612 virulence factor Mce family protein -
JTY_RS03105 693336..694478 + 1143 WP_003911253.1 virulence factor Mce family protein -
JTY_RS03110 694483..696033 + 1551 WP_003403119.1 MCE family protein -
JTY_RS03115 696085..696477 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
JTY_RS03120 696474..696731 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
JTY_RS03125 696914..698149 - 1236 WP_003403128.1 ATP-binding protein -
JTY_RS03130 698400..698813 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
JTY_RS03135 698810..699046 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JTY_RS03140 699150..699656 - 507 WP_003900973.1 two component system sensor kinase HK1 -
JTY_RS03145 699770..700300 - 531 WP_003917320.1 two component system sensor kinase HK2 -
JTY_RS03150 700284..700979 - 696 WP_197044064.1 response regulator transcription factor -
JTY_RS03155 701102..701413 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T23593 WP_003403122.1 NC_012207:c696477-696085 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T23593 NC_012207:c696477-696085 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT23593 WP_003403125.1 NC_012207:c696731-696474 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT23593 NC_012207:c696731-696474 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References