Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4710349..4711159 Replicon chromosome
Accession NZ_CP092336
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MG291_RS22665 Protein ID WP_004178461.1
Coordinates 4710349..4710882 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MG291_RS22670 Protein ID WP_002887278.1
Coordinates 4710893..4711159 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS22660 (4709180) 4709180..4710301 + 1122 WP_002887282.1 cupin domain-containing protein -
MG291_RS22665 (4710349) 4710349..4710882 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MG291_RS22670 (4710893) 4710893..4711159 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MG291_RS22675 (4711262) 4711262..4712695 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
MG291_RS22680 (4712685) 4712685..4713368 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MG291_RS22685 (4713540) 4713540..4714925 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
MG291_RS22690 (4714943) 4714943..4715287 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T235928 WP_004178461.1 NZ_CP092336:c4710882-4710349 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T235928 NZ_LR134493:c1917556-1917077 [Serratia rubidaea]
ATGATTATTCTCGATACCAACGTGATCGCAGAAACGCTGCGCCCCAAGCCGCATTACAACGTCATCAGCTGGCTGAACGA
AAAAGACAATAGCGAGCTGTACCTCAGCGCCATCGTCGTCGCCGAACTGTTTAGCGGCGTAGCCTGCATGCCGGACGGCA
AACGCCAGCAGGCGCTAAGGCTCAGGCTGGCGGAGGCGATTCAGATTAACTTTGATGAGCAGATTTTGCCGTTTGACGCC
CTGTGTGCGATGCAGTACGCGGAACTGATGGGCAGGAACCTGCGCCAGGGCACGCCGATGAGCGCGCCGGACGCGCAGAT
CGCCGCCACCTGTTTGCAGTACGGCGCCACGCTGGCCACGCGCAATACCAAAGATTTTCTCCACTGCGGCATTGAACTGA
TCGACCCCTGGCAGGTGCCCGCGGGGCAACGTCTGCATGAAGACGCGGCCGAATACTACGTGATGAGCCGTAAATCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT235928 WP_002887278.1 NZ_CP092336:c4711159-4710893 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT235928 NZ_LR134493:c1917816-1917553 [Serratia rubidaea]
ATGGCTACCATTACCGTCAGAAATCTTGACGACGAGGTCAAAGAGCTGTTGCGTATTTCGGCAGCTAAAAATGGTCATTC
CATGGAAGAGGAGGCGCGCATGATCTTAAAACAGGCGCTGATGAAAAAGCCGGCGCGTTACGGGCTGGGCAGTTGGATGC
ATCAGCACTTTGCCGAATTCGGCGGCGTAGAACTGGCGATCCCGCCGCGCGATACCGTGCCGCCGCGCCGTGTCACCTTC
GACGATGATGACGACCAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References