Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4074937..4075556 Replicon chromosome
Accession NZ_CP092336
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MG291_RS19655 Protein ID WP_002892050.1
Coordinates 4075338..4075556 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MG291_RS19650 Protein ID WP_002892066.1
Coordinates 4074937..4075311 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS19640 (4070089) 4070089..4071282 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MG291_RS19645 (4071305) 4071305..4074451 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
MG291_RS19650 (4074937) 4074937..4075311 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MG291_RS19655 (4075338) 4075338..4075556 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MG291_RS19660 (4075715) 4075715..4076281 + 567 WP_002892030.1 maltose O-acetyltransferase -
MG291_RS19665 (4076253) 4076253..4076393 - 141 WP_004147370.1 hypothetical protein -
MG291_RS19670 (4076414) 4076414..4076884 + 471 WP_002892026.1 YlaC family protein -
MG291_RS19675 (4076859) 4076859..4078310 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MG291_RS19680 (4078411) 4078411..4079109 + 699 WP_002892021.1 GNAT family protein -
MG291_RS19685 (4079106) 4079106..4079246 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MG291_RS19690 (4079246) 4079246..4079509 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4079569..4080615 1046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T235927 WP_002892050.1 NZ_CP092336:4075338-4075556 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T235927 NZ_LR134493:634675-634962 [Serratia rubidaea]
ATGACCTACAAGGTCAAATTCTGCGAGTCTGCGCTGAAAGAGTGGAACAGGCTGGATAAAGCGCTACAGCAACAGTTTGC
CAAGAAGCTGAAGAAATGCTGTGAAAATCCCCATATCCCCGCGGCGAAACTACGTGGCATGCCGGGGTGCTACAAAATCA
AGCTGCGGGCCTCTGGATTTCGGTTGGTCTATGAAGTGATCGACGATTGTTTGGTGATCGCCGTCGTTGCCGTCGGTAAG
CGGGAGCGAGGCAGCGTTTACCAATTAAGCAGCGAACGTCTGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT235927 WP_002892066.1 NZ_CP092336:4074937-4075311 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT235927 NZ_LR134493:634427-634678 [Serratia rubidaea]
GTGCCAAATATCATTCTGAGCGATACCAGCGCCAGCATCAGCGAACTGAAGAAAAACCCCATGGGGACGGTCAACGCCGG
CGAAGGGTATCCGGTCGCCATCCTCAACCGTAATCAGCCCGCATTCTATTGCGTACCGGCAGCCCTGTACGAGCAAATGC
TTGACATGCTGGATGAGCAGGAACTGGTCAGGCTGGTCAATGAGCGTCGCAATGAAGCACTGATTGACGTCGATCTGGAC
CAGTTTACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References