Detailed information of TA system TA235859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT-BrnA
• Location: 3612944..3613436
• Replicon: chromosome
• Accession: NZ_CP092283
• Organism: Marinobacter sp. M4C

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: MIH16_RS17010
• Protein ID: WP_249006001.1
• Coordinates: 3613173..3613436 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: MIH16_RS17005
• Protein ID: WP_249006000.1
• Coordinates: 3612944..3613189 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIH16_RS16975 (MIH16_16975)	3608071..3609333	+	1263	WP_249005994.1	site-specific integrase	-
MIH16_RS16980 (MIH16_16980)	3609330..3610274	+	945	WP_249005995.1	tyrosine-type recombinase/integrase	-
MIH16_RS16985 (MIH16_16985)	3610271..3611278	+	1008	WP_249005996.1	tyrosine-type recombinase/integrase	-
MIH16_RS16990 (MIH16_16990)	3611566..3612117	-	552	WP_249005997.1	hypothetical protein	-
MIH16_RS16995 (MIH16_16995)	3612119..3612484	-	366	WP_249005998.1	hypothetical protein	-
MIH16_RS17000 (MIH16_17000)	3612484..3612675	-	192	WP_249005999.1	hypothetical protein	-
MIH16_RS17005 (MIH16_17005)	3612944..3613189	-	246	WP_249006000.1	BrnA antitoxin family protein	Antitoxin
MIH16_RS17010 (MIH16_17010)	3613173..3613436	-	264	WP_249006001.1	BrnT family toxin	Toxin
MIH16_RS17015 (MIH16_17015)	3613689..3613949	-	261	WP_249006002.1	helix-turn-helix transcriptional regulator	-
MIH16_RS17020 (MIH16_17020)	3614606..3615151	-	546	WP_249006003.1	hypothetical protein	-
MIH16_RS17025 (MIH16_17025)	3615153..3617375	-	2223	WP_249006004.1	hypothetical protein	-
MIH16_RS17030 (MIH16_17030)	3617536..3617643	-	108	WP_249006005.1	hypothetical protein	-
MIH16_RS17035 (MIH16_17035)	3617769..3618215	+	447	Protein_3350	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3551811..3678945	127134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (13-77)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10262.61 Da
• Isoelectric Point: 5.8898

>T235859 WP_249006001.1 NZ_CP092283:c3613436-3613173 [Marinobacter sp. M4C]
MEIEFDNAKSERNMELRGLSFELAAKFDFDGAIEVEQEVNGEQRYFALGHIGSRLYALVYTLRTNIVRVISLRKANKREV
RRYEQFD

Nucleotide

• Length: 264 bp

>T235859 NZ_LR134442:c570823-570584 [Propionibacterium australiense]
ATGATCGAGGAAGTGCTCGAGCCGCACCGCGGGACCCGCGCCATCGTGCCATTCGACGAGCCCGCAGCCGAGCAGTACGC
CGAGGTCCTTGCCGCCGGTGAGCGCGCGGGATCACCGATCCACACCGCGGACGCCCAGATCGCAGCGATCTGCCGGGTGC
ACCGGGCGATCTGCGCCACCCGTCACACGGAGGACTTCGCCGACACCGGGATAGACCTCGTCAACCCCTGGCCGCCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9245.24 Da
• Isoelectric Point: 7.2038

>AT235859 WP_249006000.1 NZ_CP092283:c3613189-3612944 [Marinobacter sp. M4C]
MSNSIDPEMTDTDNPEWSDTMFAQAKRGTAARRVGRPKLRSPKHSTTLRIDEDVIEFFKSGGSGWQTRMNEALRQYVSEH
S

Nucleotide

• Length: 246 bp

>AT235859 NZ_LR134442:c571003-570863 [Propionibacterium australiense]
GTGATCGTCCTCGACACGAACGTCGTCTCGGAAACTCTCCGCCCCCACCCGAACGCGAGGATCGTCGCATGGCTGGAAGG
ACTCACAGACGACGTCGCCATCACCACCATCACACTCGCCGAGCTGCTCGCCGACGTGTGA