Detailed information of TA system TA235849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 543125..543762
• Replicon: chromosome
• Accession: NZ_CP092283
• Organism: Marinobacter sp. M4C

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MIH16_RS02530
• Protein ID: WP_249007417.1
• Coordinates: 543125..543535 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MIH16_RS02535
• Protein ID: WP_249007418.1
• Coordinates: 543532..543762 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIH16_RS02490 (MIH16_02490)	538469..538696	-	228	WP_249007410.1	transposase	-
MIH16_RS02495 (MIH16_02495)	538866..539012	+	147	WP_249007411.1	RRXRR domain-containing protein	-
MIH16_RS02500 (MIH16_02500)	539018..539884	+	867	WP_249007412.1	helix-turn-helix domain-containing protein	-
MIH16_RS02505 (MIH16_02505)	540264..540446	+	183	WP_249007413.1	stability determinant	-
MIH16_RS02510 (MIH16_02510)	540668..541066	+	399	WP_249007414.1	transposase	-
MIH16_RS02515 (MIH16_02515)	541063..542154	+	1092	WP_249007415.1	IS3 family transposase	-
MIH16_RS02520 (MIH16_02520)	542135..542395	-	261	Protein_502	transposase	-
MIH16_RS02525 (MIH16_02525)	542517..542945	-	429	WP_249007416.1	DUF2384 domain-containing protein	-
MIH16_RS02530 (MIH16_02530)	543125..543535	-	411	WP_249007417.1	type II toxin-antitoxin system VapC family toxin	Toxin
MIH16_RS02535 (MIH16_02535)	543532..543762	-	231	WP_249007418.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MIH16_RS02540 (MIH16_02540)	544156..545430	-	1275	WP_249007419.1	type II toxin-antitoxin system HipA family toxin	-
MIH16_RS02545 (MIH16_02545)	545427..545774	-	348	WP_249007420.1	helix-turn-helix domain-containing protein	-
MIH16_RS02550 (MIH16_02550)	546186..546428	+	243	WP_249007421.1	hypothetical protein	-
MIH16_RS02555 (MIH16_02555)	546569..546808	+	240	WP_249007422.1	hypothetical protein	-
MIH16_RS02560 (MIH16_02560)	546810..547001	-	192	WP_249007423.1	hypothetical protein	-
MIH16_RS02565 (MIH16_02565)	547077..547832	-	756	WP_249006922.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15109.29 Da
• Isoelectric Point: 6.2208

>T235849 WP_249007417.1 NZ_CP092283:c543535-543125 [Marinobacter sp. M4C]
VTTYMLDTCICSYIMREHPASVLQRLTYEVEQGDRIVISAITYAEMRYGQIGKKASAKHKVLIDEFVERVDAVLSWDQRA
VDATVTAMSQLRKAGTPIGPNDTAIAGHAIATGCTLVTNNVREFSRVPSLVYEDWV

Nucleotide

• Length: 411 bp

>T235849 NZ_LR134406:c2801120-2800866 [Arachnia propionica]
GTGCGCCTGGCGTGGGATCCCTCGGCCTGGGAGGATTACACGTACTGGCAGGCCGCGGATAGCCGGATTCTCAAGCGGAT
CAACGCGCTCATCGACACCTGCCTCCGCGATCCCTCCGGTGGGATCGGGAAACCCGAACAACTCAGGTATGGGGCACCCG
GGGCTTGGTCGCGTCGCATCACGGAGGAGCACCGGCTCGTGTATCTCGTCGACGGCGACGACCTGGTGATCCTCCAGGCC
CGGTACCACTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8397.49 Da
• Isoelectric Point: 4.9987

>AT235849 WP_249007418.1 NZ_CP092283:c543762-543532 [Marinobacter sp. M4C]
MRTVSIFKNGKNQAVRLPADMAYTGVGELEISRDGDVITLRPVRASWASFADLPKADADFLNERPVVASDESRFNL

Nucleotide

• Length: 231 bp

>AT235849 NZ_LR134406:c2801373-2801122 [Arachnia propionica]
GTGTCCATCAGCGCCAGCGAGGCCCGCAGGACGTTGTTCCCGCTCATCGAGCAGGTCAACGACGATCGCACTGCCGTCGA
GATCGTTTCCCGCAAGGGGAACGCCGTTCTCATGTCCGCCGATGAATACGCCGCGTGGCAGGAGACGGCCTACCTGTTCC
GTTCCCCCGCCAATGCCCGAAGGCTGCTCGACGCCTACGAGAGGGCCCGGGAAGGACGGAGCGAGGAACACGATCTCATC
CGGGAGGACTGA